Isolation, structural elucidation and synthesis of a tetradecapeptide with in vitro ACTH-releasing activity corresponding to residues 33-46 of the α-chain of porcine hemoglobin

Andrew V Schally, W. Y. Huang, T. W. Redding, A. Arimura, D. H. Coy, K. Chihara, R. C C Chang, V. Raymond, F. Labrie

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

A peptide found in acetic acid extracts of porcine hypothalami and capable of stimulating the release of ACTH in vitro was isolated in pure state, structurally identified as Phe-Leu-Gly-Phe-Pro-Thr-Thr-Lys-Thr-Tyr-Pre-Pro-His-Phe and synthesized. This tetradecapeptide, which corresponds to amino acid residues no. 33-46 in the sequence of the α-chain of porcine hemoglobin, probably represents an artefact of extraction or isolation procedures. Since this peptide stimulates ACTH release from rat pituitary fragments and from monolayer cultures of pituitary cells, but not in vivo, caution must be exercised in interpreting the results of in vitro assays for corticotropin releasing factor.

Original languageEnglish
Pages (from-to)582-588
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume82
Issue number2
DOIs
StatePublished - May 30 1978
Externally publishedYes

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Adrenocorticotropic Hormone
Hemoglobins
Swine
Peptides
Corticotropin-Releasing Hormone
Acetic Acid
Artifacts
Hypothalamus
Rats
Monolayers
Assays
Cell Culture Techniques
Amino Acids
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Isolation, structural elucidation and synthesis of a tetradecapeptide with in vitro ACTH-releasing activity corresponding to residues 33-46 of the α-chain of porcine hemoglobin. / Schally, Andrew V; Huang, W. Y.; Redding, T. W.; Arimura, A.; Coy, D. H.; Chihara, K.; Chang, R. C C; Raymond, V.; Labrie, F.

In: Biochemical and Biophysical Research Communications, Vol. 82, No. 2, 30.05.1978, p. 582-588.

Research output: Contribution to journalArticle

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AU - Coy, D. H.

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AU - Chang, R. C C

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