Isolation, purification and properties of tannase from Aspergillus niger van Tieghem

Shweta Sharma, T. K. Bhat, Rajinder Dawra

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Tannase (tannin acyl hydrolase E.C. 3.1.1.20) has been isolated from Aspergillus niger van Tieghem and purified 29-fold. The enzyme had a temperature optimum of 60 °C, pH optimum of 6.0 with a second peak at pH 4.5, K(m) of 0.20 mM and V(max) of 5.0 μmol min-1 mg-1 protein.

Original languageEnglish (US)
Pages (from-to)673-677
Number of pages5
JournalWorld Journal of Microbiology and Biotechnology
Volume15
Issue number6
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

Fingerprint

tannase
Aspergillus niger
Tannins
Hydrolases
Temperature
Enzymes
Proteins

Keywords

  • Aspergillus niger
  • Detanninification
  • Properties
  • Tannase

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

Cite this

Isolation, purification and properties of tannase from Aspergillus niger van Tieghem. / Sharma, Shweta; Bhat, T. K.; Dawra, Rajinder.

In: World Journal of Microbiology and Biotechnology, Vol. 15, No. 6, 01.01.1999, p. 673-677.

Research output: Contribution to journalArticle

@article{3f2e7ae8ddbf42e396ac0ec33555ab1c,
title = "Isolation, purification and properties of tannase from Aspergillus niger van Tieghem",
abstract = "Tannase (tannin acyl hydrolase E.C. 3.1.1.20) has been isolated from Aspergillus niger van Tieghem and purified 29-fold. The enzyme had a temperature optimum of 60 °C, pH optimum of 6.0 with a second peak at pH 4.5, K(m) of 0.20 mM and V(max) of 5.0 μmol min-1 mg-1 protein.",
keywords = "Aspergillus niger, Detanninification, Properties, Tannase",
author = "Shweta Sharma and Bhat, {T. K.} and Rajinder Dawra",
year = "1999",
month = "1",
day = "1",
doi = "10.1023/A:1008939816281",
language = "English (US)",
volume = "15",
pages = "673--677",
journal = "Mircen Journal of Applied Microbiology and Biotechnology",
issn = "0265-0762",
publisher = "Springer Netherlands",
number = "6",

}

TY - JOUR

T1 - Isolation, purification and properties of tannase from Aspergillus niger van Tieghem

AU - Sharma, Shweta

AU - Bhat, T. K.

AU - Dawra, Rajinder

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Tannase (tannin acyl hydrolase E.C. 3.1.1.20) has been isolated from Aspergillus niger van Tieghem and purified 29-fold. The enzyme had a temperature optimum of 60 °C, pH optimum of 6.0 with a second peak at pH 4.5, K(m) of 0.20 mM and V(max) of 5.0 μmol min-1 mg-1 protein.

AB - Tannase (tannin acyl hydrolase E.C. 3.1.1.20) has been isolated from Aspergillus niger van Tieghem and purified 29-fold. The enzyme had a temperature optimum of 60 °C, pH optimum of 6.0 with a second peak at pH 4.5, K(m) of 0.20 mM and V(max) of 5.0 μmol min-1 mg-1 protein.

KW - Aspergillus niger

KW - Detanninification

KW - Properties

KW - Tannase

UR - http://www.scopus.com/inward/record.url?scp=0032786077&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032786077&partnerID=8YFLogxK

U2 - 10.1023/A:1008939816281

DO - 10.1023/A:1008939816281

M3 - Article

AN - SCOPUS:0032786077

VL - 15

SP - 673

EP - 677

JO - Mircen Journal of Applied Microbiology and Biotechnology

JF - Mircen Journal of Applied Microbiology and Biotechnology

SN - 0265-0762

IS - 6

ER -