Isolation of xenopus oocytes

Karen Newman, Tristan Aguero, Mary Lou L King

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Xenopus oocytes and oocyte extracts are the starting material for a variety of experimental approaches. Oocytes are obtained by surgical removal of the ovary from anesthetized females. Although oocytes may be used while they remain within their ovarian follicle, it is more practical to work with defolli-culated oocytes. Defolliculation can be performed either manually or enzymatically. Here we present a protocol for the isolation and separation of Xenopus oocytes at various developmental stages, and guidelines for maintaining oocytes in culture.

Original languageEnglish (US)
Pages (from-to)86-91
Number of pages6
JournalCold Spring Harbor Protocols
Volume2018
Issue number2
DOIs
StatePublished - Feb 1 2018

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Newman, K., Aguero, T., & King, M. L. L. (2018). Isolation of xenopus oocytes. Cold Spring Harbor Protocols, 2018(2), 86-91. https://doi.org/10.1101/pdb.prot095851