Isolation of plasma membrane glycoprotein from bovine thymocytes

M. A. Fletcher, T. M. Lo, W. R. Graves, A. M. Robles

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Glycoprotein was isolated from a purified thymocyte membrane preparation by two methods: lithium diiodosalicylate-phenol extraction and hot 75% ethanol extraction. A higher yield of membrane sialic acid was obtained by the latter method. The preparations had similar apparent molecular weights on sodium dodecyl sulfate gel electrophoresis. Both had similar receptor activities against a panel of hemagglutinins, although the 75% ethanol extract was more active on a weight basis. However, there were significant differences in carbohydrate and amino acid compositions of the two thymocyte extracts. The lithium diiodosalicylate-extracted material had much more glucose, ribose, and glycine than the ethanol extract. The glycoprotein preparations from thymocytes were quite distinct from the glycoprotein of bovine erythrocytes in both composition and receptor properties.

Original languageEnglish (US)
Pages (from-to)341-352
Number of pages12
JournalBBA - Biomembranes
Volume465
Issue number2
DOIs
StatePublished - Mar 1 1977

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Isolation of plasma membrane glycoprotein from bovine thymocytes'. Together they form a unique fingerprint.

  • Cite this

    Fletcher, M. A., Lo, T. M., Graves, W. R., & Robles, A. M. (1977). Isolation of plasma membrane glycoprotein from bovine thymocytes. BBA - Biomembranes, 465(2), 341-352. https://doi.org/10.1016/0005-2736(77)90083-9