Isolation of pancreatic islets from nonhuman primates

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Nonhuman primates (NHP) constitute a highly relevant pre-clinical animal model to develop strategies for beta cell replacement. The close phylogenetic and immunologic relationship between NHP and humans results in cross-reactivity of various biological agents with NHP cells, as well as a very similar cytoarchitecture between islets from human and NHP that is strikingly different from that observed in rodent islets. The composition and location of endocrine cells in human or NHP islets, randomly distributed and associated with blood vessels, have functional consequences and a predisposition for paracrine interactions. Furthermore, translation of approaches that proved successful in rodent models to the clinic has been limited. Consequently, data collected from NHP studies can form the basis for an IND submission to the FDA. This chapter describes in detail the key aspects for isolation of islets from NHP, from organ procurement up to assessment of islet function, comparing and emphasizing the similarities between isolation procedures for human and NHP islets.

Original languageEnglish (US)
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York LLC
Pages57-66
Number of pages10
Volume938
DOIs
StatePublished - 2016

Publication series

NameAdvances in Experimental Medicine and Biology
Volume938
ISSN (Print)00652598
ISSN (Electronic)22148019

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Keywords

  • Islet assessment
  • Islet isolation
  • Islet purifi cation
  • Islet yield

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Berman-Weinberg, D. (2016). Isolation of pancreatic islets from nonhuman primates. In Advances in Experimental Medicine and Biology (Vol. 938, pp. 57-66). (Advances in Experimental Medicine and Biology; Vol. 938). Springer New York LLC. https://doi.org/10.1007/978-3-319-39824-2_5