Abstract
We describe a procedure for the isolation of large numbers of canine pancreatic islets with a high level of purity. The method utilizes enzymatic and mechanical disruption of the pancreas, followed by islet purification on discontinuous density gradients of bovine serum albumin. Purification has been semiautomated through use of the Cobe 2991 cell processor. Islet preparations have a purity of 86.5% and contain 36.8 μl of endocrine tissue. Islets isolated by this procedure are functionally intact, and restore fasting euglycemia following auto-transplantation.
| Original language | English |
|---|---|
| Pages (from-to) | 207-210 |
| Number of pages | 4 |
| Journal | Transplantation |
| Volume | 50 |
| Issue number | 2 |
| State | Published - Jan 1 1990 |
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ASJC Scopus subject areas
- Immunology
- Transplantation
Cite this
Isolation of pancreatic islets from dogs. Semiautomated purification on albumin gradients. / Alejandro, Rodolfo; Strasser, S.; Zucker, P. F.; Mintz, D. H.
In: Transplantation, Vol. 50, No. 2, 01.01.1990, p. 207-210.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation of pancreatic islets from dogs. Semiautomated purification on albumin gradients
AU - Alejandro, Rodolfo
AU - Strasser, S.
AU - Zucker, P. F.
AU - Mintz, D. H.
PY - 1990/1/1
Y1 - 1990/1/1
N2 - We describe a procedure for the isolation of large numbers of canine pancreatic islets with a high level of purity. The method utilizes enzymatic and mechanical disruption of the pancreas, followed by islet purification on discontinuous density gradients of bovine serum albumin. Purification has been semiautomated through use of the Cobe 2991 cell processor. Islet preparations have a purity of 86.5% and contain 36.8 μl of endocrine tissue. Islets isolated by this procedure are functionally intact, and restore fasting euglycemia following auto-transplantation.
AB - We describe a procedure for the isolation of large numbers of canine pancreatic islets with a high level of purity. The method utilizes enzymatic and mechanical disruption of the pancreas, followed by islet purification on discontinuous density gradients of bovine serum albumin. Purification has been semiautomated through use of the Cobe 2991 cell processor. Islet preparations have a purity of 86.5% and contain 36.8 μl of endocrine tissue. Islets isolated by this procedure are functionally intact, and restore fasting euglycemia following auto-transplantation.
UR - http://www.scopus.com/inward/record.url?scp=0024999097&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024999097&partnerID=8YFLogxK
M3 - Article
C2 - 1696406
AN - SCOPUS:0024999097
VL - 50
SP - 207
EP - 210
JO - Transplantation
JF - Transplantation
SN - 0041-1337
IS - 2
ER -