Isolation of pancreatic islets from dogs. Semiautomated purification on albumin gradients

Rodolfo Alejandro, S. Strasser, P. F. Zucker, D. H. Mintz

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

We describe a procedure for the isolation of large numbers of canine pancreatic islets with a high level of purity. The method utilizes enzymatic and mechanical disruption of the pancreas, followed by islet purification on discontinuous density gradients of bovine serum albumin. Purification has been semiautomated through use of the Cobe 2991 cell processor. Islet preparations have a purity of 86.5% and contain 36.8 μl of endocrine tissue. Islets isolated by this procedure are functionally intact, and restore fasting euglycemia following auto-transplantation.

Original languageEnglish
Pages (from-to)207-210
Number of pages4
JournalTransplantation
Volume50
Issue number2
StatePublished - Jan 1 1990

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Islets of Langerhans
Albumins
Dogs
Bovine Serum Albumin
Canidae
Pancreas
Fasting
Transplantation

ASJC Scopus subject areas

  • Immunology
  • Transplantation

Cite this

Isolation of pancreatic islets from dogs. Semiautomated purification on albumin gradients. / Alejandro, Rodolfo; Strasser, S.; Zucker, P. F.; Mintz, D. H.

In: Transplantation, Vol. 50, No. 2, 01.01.1990, p. 207-210.

Research output: Contribution to journalArticle

Alejandro, R, Strasser, S, Zucker, PF & Mintz, DH 1990, 'Isolation of pancreatic islets from dogs. Semiautomated purification on albumin gradients', Transplantation, vol. 50, no. 2, pp. 207-210.
Alejandro, Rodolfo ; Strasser, S. ; Zucker, P. F. ; Mintz, D. H. / Isolation of pancreatic islets from dogs. Semiautomated purification on albumin gradients. In: Transplantation. 1990 ; Vol. 50, No. 2. pp. 207-210.
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