Isolation of pancreatic islets from dogs: Semiautomated purification on albumin gradients

Rodolfo Alejandro, Sheryl Strasser, Peter F. Zucker, Daniel H. Mintz

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

We describe a procedure for the isolation of large numbers of canine pancreatic islets with a high level of purity. The method utilizes enzymatic and mechanical disruption of the pancreas, followed by islet purification on discontinuous density gradients of bovine serum albumin. Purification has been semiautomated through use of the Cobe 2991 cell processor. Islet preparations have a purity of 86.5% and contain 36.8 μl of endocrine tissue. Islets isolated by this procedure are functionally intact, and restore fasting euglycemia following auto-transplantation.

Original languageEnglish (US)
Pages (from-to)207-210
Number of pages4
JournalTransplantation
Volume50
Issue number2
DOIs
StatePublished - Aug 1990

ASJC Scopus subject areas

  • Transplantation

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