Abstract
A human retina cDNA library enriched for retina-specific clones was prepared by subtraction with a non-retina population of cDNA in combination with polymerase chain reaction (PCR) amplifications. A highly retina-specific cDNA clone (1190 bp) was obtained through this library encoding a 200 amino acid protein with three calcium binding sites and 87% homology to the bovine photoreceptor protein, recoverin, which has been shown to mediate the recovery of the dark current after photoactivation, and 58% homology to the calcium-binding chick cone protein, visinin. Analysis of the gene indicated a 9-10 kb single-copy gene with at least three exons and two introns. The three exons contained the entire coding sequence, and all of the calcium-binding EF-hand regions were in putative exon 1. The recoverin gene was mapped to human chromosome 17 by hybridization to a panel of human-rodent hybrid DNAs.
| Original language | English |
|---|---|
| Pages (from-to) | 234-244 |
| Number of pages | 11 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 187 |
| Issue number | 1 |
| DOIs | |
| State | Published - Aug 31 1992 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Isolation of human retinal genes : Recoverin cDNA and gene. / Murakami, Akira; Yajima, Toshihiro; Inana, George.
In: Biochemical and Biophysical Research Communications, Vol. 187, No. 1, 31.08.1992, p. 234-244.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation of human retinal genes
T2 - Recoverin cDNA and gene
AU - Murakami, Akira
AU - Yajima, Toshihiro
AU - Inana, George
PY - 1992/8/31
Y1 - 1992/8/31
N2 - A human retina cDNA library enriched for retina-specific clones was prepared by subtraction with a non-retina population of cDNA in combination with polymerase chain reaction (PCR) amplifications. A highly retina-specific cDNA clone (1190 bp) was obtained through this library encoding a 200 amino acid protein with three calcium binding sites and 87% homology to the bovine photoreceptor protein, recoverin, which has been shown to mediate the recovery of the dark current after photoactivation, and 58% homology to the calcium-binding chick cone protein, visinin. Analysis of the gene indicated a 9-10 kb single-copy gene with at least three exons and two introns. The three exons contained the entire coding sequence, and all of the calcium-binding EF-hand regions were in putative exon 1. The recoverin gene was mapped to human chromosome 17 by hybridization to a panel of human-rodent hybrid DNAs.
AB - A human retina cDNA library enriched for retina-specific clones was prepared by subtraction with a non-retina population of cDNA in combination with polymerase chain reaction (PCR) amplifications. A highly retina-specific cDNA clone (1190 bp) was obtained through this library encoding a 200 amino acid protein with three calcium binding sites and 87% homology to the bovine photoreceptor protein, recoverin, which has been shown to mediate the recovery of the dark current after photoactivation, and 58% homology to the calcium-binding chick cone protein, visinin. Analysis of the gene indicated a 9-10 kb single-copy gene with at least three exons and two introns. The three exons contained the entire coding sequence, and all of the calcium-binding EF-hand regions were in putative exon 1. The recoverin gene was mapped to human chromosome 17 by hybridization to a panel of human-rodent hybrid DNAs.
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UR - http://www.scopus.com/inward/citedby.url?scp=0026687303&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(05)81483-4
DO - 10.1016/S0006-291X(05)81483-4
M3 - Article
C2 - 1387789
AN - SCOPUS:0026687303
VL - 187
SP - 234
EP - 244
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -