Isolation of human retinal genes: Recoverin cDNA and gene

Akira Murakami, Toshihiro Yajima, George Inana

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

A human retina cDNA library enriched for retina-specific clones was prepared by subtraction with a non-retina population of cDNA in combination with polymerase chain reaction (PCR) amplifications. A highly retina-specific cDNA clone (1190 bp) was obtained through this library encoding a 200 amino acid protein with three calcium binding sites and 87% homology to the bovine photoreceptor protein, recoverin, which has been shown to mediate the recovery of the dark current after photoactivation, and 58% homology to the calcium-binding chick cone protein, visinin. Analysis of the gene indicated a 9-10 kb single-copy gene with at least three exons and two introns. The three exons contained the entire coding sequence, and all of the calcium-binding EF-hand regions were in putative exon 1. The recoverin gene was mapped to human chromosome 17 by hybridization to a panel of human-rodent hybrid DNAs.

Original languageEnglish
Pages (from-to)234-244
Number of pages11
JournalBiochemical and Biophysical Research Communications
Volume187
Issue number1
DOIs
StatePublished - Aug 31 1992

Fingerprint

Recoverin
Retina
Exons
Complementary DNA
Genes
Calcium
Clone Cells
EF Hand Motifs
Chromosomes, Human, Pair 17
Proteins
Dark currents
Polymerase chain reaction
Human Chromosomes
Chromosomes
Gene Library
Introns
Libraries
Amplification
Cones
Rodentia

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Isolation of human retinal genes : Recoverin cDNA and gene. / Murakami, Akira; Yajima, Toshihiro; Inana, George.

In: Biochemical and Biophysical Research Communications, Vol. 187, No. 1, 31.08.1992, p. 234-244.

Research output: Contribution to journalArticle

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