Isolation of human NURF: A regulator of Engrailed gene expression

Orr Barak, Maribeth A. Lazzaro, William S. Lane, David W. Speicher, David J. Picketts, Ramin Shiekhattar

Research output: Contribution to journalArticle

116 Scopus citations

Abstract

The modification of chromatin structure is an important regulatory mechanism for developmental gene expression. Differential expression of the mammalian ISWI genes, SNF2H and SNF2L, has suggested that they possess distinct developmental roles. Here we describe the purification and characterization of the first human SNF2L-containing complex. The subunit composition suggests that it represents the human ortholog of the Drosophila nucleosome-remodeling factor (NURF) complex. Human NURF (hNURF) is enriched in brain, and we demonstrate that it regulates human Engrailed, a homeodomain protein that regulates neuronal development in the mid-hindbrain. Furthermore, we show that hNURF potentiates neurite outgrowth in cell culture. Taken together, our data suggess a role for an ISWI complex in neuronal growth.

Original languageEnglish (US)
Pages (from-to)6089-6100
Number of pages12
JournalEMBO Journal
Volume22
Issue number22
DOIs
StatePublished - Nov 17 2003

Keywords

  • BPTF
  • Engrailed
  • ISWI
  • NURF
  • SNF2L

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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    Barak, O., Lazzaro, M. A., Lane, W. S., Speicher, D. W., Picketts, D. J., & Shiekhattar, R. (2003). Isolation of human NURF: A regulator of Engrailed gene expression. EMBO Journal, 22(22), 6089-6100. https://doi.org/10.1093/emboj/cdg582