Isolation of high spectral quality RNA using run-on gene transcription; application to gene expression profiling of human brain

Jian Guo Cui, Yuhai Zhao, Walter J. Lukiw

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

1. Gene expression studies of human brain tissues in both health and disease require the use of intact, high spectral quality messenger RNA (mRNA). Short post-mortem tissues, when adequately controlled, provide a valuable primary source of brain mRNA. 2. Typical mRNA isolation methodologies rely on total RNA extraction from whole cells or tissues. Here we report that as an alternative approach, nuclei can be isolated from the same tissues and programmed for run-on gene transcription to generate enriched mRNA fractions. 3. This novel technique represents a significant improvement over previous methods and provides high quality, high yield mRNA samples suitable for downstream applications that include cloning, sequencing and gene expression studies using DNA array technologies.

Original languageEnglish
Pages (from-to)789-794
Number of pages6
JournalCellular and Molecular Neurobiology
Volume25
Issue number3-4
DOIs
StatePublished - Jun 1 2005

Fingerprint

Gene Expression Profiling
Transcription
Gene expression
Brain
Genes
RNA
Messenger RNA
Tissue
Gene Expression
Cloning
Oligonucleotide Array Sequence Analysis
Organism Cloning
Health
Technology
DNA

Keywords

  • Alzheimer's disease (AD)
  • Brain transcription
  • Messenger RNA
  • Micro-machines
  • Nanotechnology
  • RNA polymerase II
  • Run-on gene transcription

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Biochemistry
  • Cell Biology
  • Genetics

Cite this

Isolation of high spectral quality RNA using run-on gene transcription; application to gene expression profiling of human brain. / Cui, Jian Guo; Zhao, Yuhai; Lukiw, Walter J.

In: Cellular and Molecular Neurobiology, Vol. 25, No. 3-4, 01.06.2005, p. 789-794.

Research output: Contribution to journalArticle

@article{d0616f9511d24fe7baec5b6c63f80b49,
title = "Isolation of high spectral quality RNA using run-on gene transcription; application to gene expression profiling of human brain",
abstract = "1. Gene expression studies of human brain tissues in both health and disease require the use of intact, high spectral quality messenger RNA (mRNA). Short post-mortem tissues, when adequately controlled, provide a valuable primary source of brain mRNA. 2. Typical mRNA isolation methodologies rely on total RNA extraction from whole cells or tissues. Here we report that as an alternative approach, nuclei can be isolated from the same tissues and programmed for run-on gene transcription to generate enriched mRNA fractions. 3. This novel technique represents a significant improvement over previous methods and provides high quality, high yield mRNA samples suitable for downstream applications that include cloning, sequencing and gene expression studies using DNA array technologies.",
keywords = "Alzheimer's disease (AD), Brain transcription, Messenger RNA, Micro-machines, Nanotechnology, RNA polymerase II, Run-on gene transcription",
author = "Cui, {Jian Guo} and Yuhai Zhao and Lukiw, {Walter J.}",
year = "2005",
month = "6",
day = "1",
doi = "10.1007/s10571-005-4035-x",
language = "English",
volume = "25",
pages = "789--794",
journal = "Cellular and Molecular Neurobiology",
issn = "0272-4340",
publisher = "Springer New York",
number = "3-4",

}

TY - JOUR

T1 - Isolation of high spectral quality RNA using run-on gene transcription; application to gene expression profiling of human brain

AU - Cui, Jian Guo

AU - Zhao, Yuhai

AU - Lukiw, Walter J.

PY - 2005/6/1

Y1 - 2005/6/1

N2 - 1. Gene expression studies of human brain tissues in both health and disease require the use of intact, high spectral quality messenger RNA (mRNA). Short post-mortem tissues, when adequately controlled, provide a valuable primary source of brain mRNA. 2. Typical mRNA isolation methodologies rely on total RNA extraction from whole cells or tissues. Here we report that as an alternative approach, nuclei can be isolated from the same tissues and programmed for run-on gene transcription to generate enriched mRNA fractions. 3. This novel technique represents a significant improvement over previous methods and provides high quality, high yield mRNA samples suitable for downstream applications that include cloning, sequencing and gene expression studies using DNA array technologies.

AB - 1. Gene expression studies of human brain tissues in both health and disease require the use of intact, high spectral quality messenger RNA (mRNA). Short post-mortem tissues, when adequately controlled, provide a valuable primary source of brain mRNA. 2. Typical mRNA isolation methodologies rely on total RNA extraction from whole cells or tissues. Here we report that as an alternative approach, nuclei can be isolated from the same tissues and programmed for run-on gene transcription to generate enriched mRNA fractions. 3. This novel technique represents a significant improvement over previous methods and provides high quality, high yield mRNA samples suitable for downstream applications that include cloning, sequencing and gene expression studies using DNA array technologies.

KW - Alzheimer's disease (AD)

KW - Brain transcription

KW - Messenger RNA

KW - Micro-machines

KW - Nanotechnology

KW - RNA polymerase II

KW - Run-on gene transcription

UR - http://www.scopus.com/inward/record.url?scp=23944507076&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23944507076&partnerID=8YFLogxK

U2 - 10.1007/s10571-005-4035-x

DO - 10.1007/s10571-005-4035-x

M3 - Article

VL - 25

SP - 789

EP - 794

JO - Cellular and Molecular Neurobiology

JF - Cellular and Molecular Neurobiology

SN - 0272-4340

IS - 3-4

ER -