Isolation of genomic DNA from mammalian cells.

John Gilbert, Jeffery M Vance

Research output: Contribution to journal › Article

3 Citations (Scopus)

Abstract

This unit describes simple, cost-effective preparation of DNA from whole blood or cultured cells that yields high-molecular-weight DNA suitable for both Southern blotting and the polymerase chain reaction. Preparation time may be shortened by substituting a high-salt precipitation procedure for the dialysis step; however, this results in a smaller average fragment size. The isolation of DNA from buccal swabs, collected from the inside of the cheek, is also described. The DNA is suitable for PCR analysis. Preparation of buffered phenol for DNA extraction is described in a support protocol. This unit describes simple, cost-effective preparation of DNA from whole blood or cultured cells that yields high-molecular-we.

Original languageEnglish
JournalCurrent protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]
VolumeAppendix 3
StatePublished - May 1 2001
Externally publishedYes

Fingerprint

DNA
Cheek
Cultured Cells
Blood Cells
Costs and Cost Analysis
Polymerase Chain Reaction
Southern Blotting
Phenol
Dialysis
Salts
Molecular Weight

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Isolation of genomic DNA from mammalian cells. / Gilbert, John; Vance, Jeffery M.

In: Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.], Vol. Appendix 3, 01.05.2001.

Research output: Contribution to journal › Article

Gilbert, J & Vance, JM 2001, 'Isolation of genomic DNA from mammalian cells.', Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.], vol. Appendix 3.
Gilbert J, Vance JM. Isolation of genomic DNA from mammalian cells. Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]. 2001 May 1;Appendix 3.
Gilbert, John ; Vance, Jeffery M. / Isolation of genomic DNA from mammalian cells. In: Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]. 2001 ; Vol. Appendix 3.
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