Isolation of colony stimulating factor from human milk

Sudhir K. Sinha, Adel A. Yunis

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


Human milk contains colony stimulating factor (CSF), a polypeptide growth factor, which stimulates in invitro bone marrow culture proliferation and differentiation of colony forming granulocytic macrophage progenitor cells (CFU-GM) to form colonies. This activity was not found in either bovine milk or colostrum when assayed in human or mouse bone marrow cells. The human milk CSF activity is destroyed by treatment with proteases. However, neither 6M urea, 4M guanidine hydrochloride, 5 mM dithiothreitol, nor exposure to pH 2 will inactivate the milk derived CSF. Gel filtration and isoelectric focusing indicate that human milk CSF differs biochemically from the other CSFs isolated from various sources and has a molecular weight between 250,000 and 240,000 and an isoelectric point between 4.4 and 4.9.

Original languageEnglish (US)
Pages (from-to)797-803
Number of pages7
JournalBiochemical and biophysical research communications
Issue number2
StatePublished - Jul 29 1983

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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