TY - JOUR
T1 - Isolation of colony stimulating factor from human milk
AU - Sinha, Sudhir K.
AU - Yunis, Adel A.
PY - 1983/7/29
Y1 - 1983/7/29
N2 - Human milk contains colony stimulating factor (CSF), a polypeptide growth factor, which stimulates in invitro bone marrow culture proliferation and differentiation of colony forming granulocytic macrophage progenitor cells (CFU-GM) to form colonies. This activity was not found in either bovine milk or colostrum when assayed in human or mouse bone marrow cells. The human milk CSF activity is destroyed by treatment with proteases. However, neither 6M urea, 4M guanidine hydrochloride, 5 mM dithiothreitol, nor exposure to pH 2 will inactivate the milk derived CSF. Gel filtration and isoelectric focusing indicate that human milk CSF differs biochemically from the other CSFs isolated from various sources and has a molecular weight between 250,000 and 240,000 and an isoelectric point between 4.4 and 4.9.
AB - Human milk contains colony stimulating factor (CSF), a polypeptide growth factor, which stimulates in invitro bone marrow culture proliferation and differentiation of colony forming granulocytic macrophage progenitor cells (CFU-GM) to form colonies. This activity was not found in either bovine milk or colostrum when assayed in human or mouse bone marrow cells. The human milk CSF activity is destroyed by treatment with proteases. However, neither 6M urea, 4M guanidine hydrochloride, 5 mM dithiothreitol, nor exposure to pH 2 will inactivate the milk derived CSF. Gel filtration and isoelectric focusing indicate that human milk CSF differs biochemically from the other CSFs isolated from various sources and has a molecular weight between 250,000 and 240,000 and an isoelectric point between 4.4 and 4.9.
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U2 - 10.1016/0006-291X(83)90852-5
DO - 10.1016/0006-291X(83)90852-5
M3 - Article
C2 - 6349636
AN - SCOPUS:0020959626
VL - 114
SP - 797
EP - 803
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -