Isolation of c-Kit+ human amniotic fluid stem cells from second trimester

Michela Pozzobon, Martina Piccoli, Andrea Alex Schiavo, Anthony Atala, Paolo De Coppi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Amniotic fl uid-derived stem (AFS) cells have been described as an appealing source of stem cells because of their (1) fetal, non-embryonic origin, (2) easy access during pregnancy overcoming the ethical issues related both to the use of human embryonic cells and to the postnatal tissue biopsy with donor site morbidity, and (3) their undemanding ability to be expanded. We and others have demonstrated the broad differentiation potential and here we describe the established protocol we developed to obtain c-Kit+ human AFS cells, starting from second trimester amniocentesis samples.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages191-198
Number of pages8
Volume1035
DOIs
StatePublished - Dec 1 2013

Publication series

NameMethods in Molecular Biology
Volume1035
ISSN (Print)10643745

Fingerprint

Second Pregnancy Trimester
Amniotic Fluid
Stem Cells
Amniocentesis
Ethics
Morbidity
Biopsy
Pregnancy

Keywords

  • Amnioticfluid stem cells
  • C-Kit
  • CD117
  • Fetal cells
  • Human stem cells
  • Magnetic selection

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Pozzobon, M., Piccoli, M., Schiavo, A. A., Atala, A., & De Coppi, P. (2013). Isolation of c-Kit+ human amniotic fluid stem cells from second trimester. In Methods in Molecular Biology (Vol. 1035, pp. 191-198). (Methods in Molecular Biology; Vol. 1035). https://doi.org/10.1007/978-1-62703-508-8-16

Isolation of c-Kit+ human amniotic fluid stem cells from second trimester. / Pozzobon, Michela; Piccoli, Martina; Schiavo, Andrea Alex; Atala, Anthony; De Coppi, Paolo.

Methods in Molecular Biology. Vol. 1035 2013. p. 191-198 (Methods in Molecular Biology; Vol. 1035).

Research output: Chapter in Book/Report/Conference proceedingChapter

Pozzobon, M, Piccoli, M, Schiavo, AA, Atala, A & De Coppi, P 2013, Isolation of c-Kit+ human amniotic fluid stem cells from second trimester. in Methods in Molecular Biology. vol. 1035, Methods in Molecular Biology, vol. 1035, pp. 191-198. https://doi.org/10.1007/978-1-62703-508-8-16
Pozzobon M, Piccoli M, Schiavo AA, Atala A, De Coppi P. Isolation of c-Kit+ human amniotic fluid stem cells from second trimester. In Methods in Molecular Biology. Vol. 1035. 2013. p. 191-198. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-508-8-16
Pozzobon, Michela ; Piccoli, Martina ; Schiavo, Andrea Alex ; Atala, Anthony ; De Coppi, Paolo. / Isolation of c-Kit+ human amniotic fluid stem cells from second trimester. Methods in Molecular Biology. Vol. 1035 2013. pp. 191-198 (Methods in Molecular Biology).
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