Isolation of c-Kit+ human amniotic fluid stem cells from second trimester

Michela Pozzobon, Martina Piccoli, Andrea Alex Schiavo, Anthony Atala, Paolo De Coppi

Research output: Chapter in Book/Report/Conference proceedingChapter

15 Scopus citations


Amniotic fl uid-derived stem (AFS) cells have been described as an appealing source of stem cells because of their (1) fetal, non-embryonic origin, (2) easy access during pregnancy overcoming the ethical issues related both to the use of human embryonic cells and to the postnatal tissue biopsy with donor site morbidity, and (3) their undemanding ability to be expanded. We and others have demonstrated the broad differentiation potential and here we describe the established protocol we developed to obtain c-Kit+ human AFS cells, starting from second trimester amniocentesis samples.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages8
ISBN (Print)9781627035071
StatePublished - 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)10643745



  • Amnioticfluid stem cells
  • C-Kit
  • CD117
  • Fetal cells
  • Human stem cells
  • Magnetic selection

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Pozzobon, M., Piccoli, M., Schiavo, A. A., Atala, A., & De Coppi, P. (2013). Isolation of c-Kit+ human amniotic fluid stem cells from second trimester. In Methods in Molecular Biology (Vol. 1035, pp. 191-198). (Methods in Molecular Biology; Vol. 1035). Humana Press Inc..