Isolation of c-Kit+ human amniotic fluid stem cells from second trimester

Michela Pozzobon, Martina Piccoli, Andrea Alex Schiavo, Anthony Atala, Paolo De Coppi

Research output: Chapter in Book/Report/Conference proceedingChapter

16 Scopus citations

Abstract

Amniotic fl uid-derived stem (AFS) cells have been described as an appealing source of stem cells because of their (1) fetal, non-embryonic origin, (2) easy access during pregnancy overcoming the ethical issues related both to the use of human embryonic cells and to the postnatal tissue biopsy with donor site morbidity, and (3) their undemanding ability to be expanded. We and others have demonstrated the broad differentiation potential and here we describe the established protocol we developed to obtain c-Kit+ human AFS cells, starting from second trimester amniocentesis samples.

Original languageEnglish (US)
Title of host publicationStem Cell Niche
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages191-198
Number of pages8
ISBN (Print)9781627035071
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1035
ISSN (Print)1064-3745

Keywords

  • Amnioticfluid stem cells
  • C-Kit
  • CD117
  • Fetal cells
  • Human stem cells
  • Magnetic selection

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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