Isolation of amniotic stem cell lines with potential for therapy

Paolo De Coppi, Georg Bartsch, M. Minhaj Siddiqui, Tao Xu, Cesar C. Santos, Laura Perin, Gustavo Mostoslavsky, Angéline C. Serre, Evan Y. Snyder, James J. Yoo, Mark E. Furth, Shay Soker, Anthony Atala

Research output: Contribution to journalArticle

1338 Citations (Scopus)

Abstract

Stem cells capable of differentiating to multiple lineages may be valuable for therapy. We report the isolation of human and rodent amniotic fluid-derived stem (AFS) cells that express embryonic and adult stem cell markers. Undifferentiated AFS cells expand extensively without feeders, double in 36 h and are not tumorigenic. Lines maintained for over 250 population doublings retained long telomeres and a normal karyotype. AFS cells are broadly multipotent. Clonal human lines verified by retroviral marking were induced to differentiate into cell types representing each embryonic germ layer, including cells of adipogenic, osteogenic, myogenic, endothelial, neuronal and hepatic lineages. Examples of differentiated cells derived from human AFS cells and displaying specialized functions include neuronal lineage cells secreting the neurotransmitter L-glutamate or expressing G-protein-gated inwardly rectifying potassium channels, hepatic lineage cells producing urea, and osteogenic lineage cells forming tissue-engineered bone.

Original languageEnglish
Pages (from-to)100-106
Number of pages7
JournalNature Biotechnology
Volume25
Issue number1
DOIs
StatePublished - Jan 5 2007
Externally publishedYes

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Stem cells
Amniotic Fluid
Stem Cells
Cell Line
Fluids
Multipotent Stem Cells
Inwardly Rectifying Potassium Channel
Therapeutics
Germ Layers
Adult Stem Cells
Telomere
Embryonic Stem Cells
Karyotype
GTP-Binding Proteins
Neurotransmitter Agents
Urea
Glutamic Acid
Hepatocytes
Rodentia
Potassium

ASJC Scopus subject areas

  • Microbiology

Cite this

De Coppi, P., Bartsch, G., Siddiqui, M. M., Xu, T., Santos, C. C., Perin, L., ... Atala, A. (2007). Isolation of amniotic stem cell lines with potential for therapy. Nature Biotechnology, 25(1), 100-106. https://doi.org/10.1038/nbt1274

Isolation of amniotic stem cell lines with potential for therapy. / De Coppi, Paolo; Bartsch, Georg; Siddiqui, M. Minhaj; Xu, Tao; Santos, Cesar C.; Perin, Laura; Mostoslavsky, Gustavo; Serre, Angéline C.; Snyder, Evan Y.; Yoo, James J.; Furth, Mark E.; Soker, Shay; Atala, Anthony.

In: Nature Biotechnology, Vol. 25, No. 1, 05.01.2007, p. 100-106.

Research output: Contribution to journalArticle

De Coppi, P, Bartsch, G, Siddiqui, MM, Xu, T, Santos, CC, Perin, L, Mostoslavsky, G, Serre, AC, Snyder, EY, Yoo, JJ, Furth, ME, Soker, S & Atala, A 2007, 'Isolation of amniotic stem cell lines with potential for therapy', Nature Biotechnology, vol. 25, no. 1, pp. 100-106. https://doi.org/10.1038/nbt1274
De Coppi P, Bartsch G, Siddiqui MM, Xu T, Santos CC, Perin L et al. Isolation of amniotic stem cell lines with potential for therapy. Nature Biotechnology. 2007 Jan 5;25(1):100-106. https://doi.org/10.1038/nbt1274
De Coppi, Paolo ; Bartsch, Georg ; Siddiqui, M. Minhaj ; Xu, Tao ; Santos, Cesar C. ; Perin, Laura ; Mostoslavsky, Gustavo ; Serre, Angéline C. ; Snyder, Evan Y. ; Yoo, James J. ; Furth, Mark E. ; Soker, Shay ; Atala, Anthony. / Isolation of amniotic stem cell lines with potential for therapy. In: Nature Biotechnology. 2007 ; Vol. 25, No. 1. pp. 100-106.
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