Isolation of a guanylate cyclase inhibitor from the balsam pear (Momordica charantia abreviata)

David L. Vesely; William R. Graves; Timothy M. Lo; Mary Ann Fletcher; Gerald S. Levey

doi:10.1016/S0006-291X(77)80120-4

Isolation of a guanylate cyclase inhibitor from the balsam pear (Momordica charantia abreviata)

David L. Vesely, William R. Graves, Timothy M. Lo, Mary Ann Fletcher, Gerald S. Levey

Medicine

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

The balsam pear (Momordica charantia abreviata) is a plant which grows wild throughout subtropical regions including the Gulf Coast and Florida. The ripe fruit is highly toxic and produces mild hypoglycemia. Preparations of the ripe fruit and the leaves but not the unripe fruit or seeds inhibited guanylate cyclase activity whereas adenylate cyclase activity was unaffected. The guanylate cyclase inhibitor (GCI) has been purified about 30-fold has an estimated molecular weight of 5000 to 50,000 and is acid stable and heat labile. GCI blocked the activation of guanylate cyclase by nitroso chemical carcinogens, and therefore, may be useful in studying mechanisms of chemical carcinogenesis.

Original language	English (US)
Pages (from-to)	1294-1299
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	77
Issue number	4
DOIs	https://doi.org/10.1016/S0006-291X(77)80120-4
State	Published - Aug 22 1977

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/S0006-291X(77)80120-4

Fingerprint Dive into the research topics of 'Isolation of a guanylate cyclase inhibitor from the balsam pear (Momordica charantia abreviata)'. Together they form a unique fingerprint.

Cite this

@article{80e4203b707c41e3a2bc7c71d88e9107,

title = "Isolation of a guanylate cyclase inhibitor from the balsam pear (Momordica charantia abreviata)",

abstract = "The balsam pear (Momordica charantia abreviata) is a plant which grows wild throughout subtropical regions including the Gulf Coast and Florida. The ripe fruit is highly toxic and produces mild hypoglycemia. Preparations of the ripe fruit and the leaves but not the unripe fruit or seeds inhibited guanylate cyclase activity whereas adenylate cyclase activity was unaffected. The guanylate cyclase inhibitor (GCI) has been purified about 30-fold has an estimated molecular weight of 5000 to 50,000 and is acid stable and heat labile. GCI blocked the activation of guanylate cyclase by nitroso chemical carcinogens, and therefore, may be useful in studying mechanisms of chemical carcinogenesis.",

author = "Vesely, {David L.} and Graves, {William R.} and Lo, {Timothy M.} and Fletcher, {Mary Ann} and Levey, {Gerald S.}",

note = "Funding Information: We thank Julia F. Morton who stimulated our interest in studying the balsam pear. This work was supported by NIH grant HL 13715-07 and USPHS AM-167-63-06. Dr. Levey is an Investigator of Howard Hughes Medical Institute. This work was presented at the 59th Annual Meeting of the Endocrine Society, Chicago, lllinois, June 8, 1977. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1977",

month = aug,

day = "22",

doi = "10.1016/S0006-291X(77)80120-4",

language = "English (US)",

volume = "77",

pages = "1294--1299",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "4",

}

TY - JOUR

T1 - Isolation of a guanylate cyclase inhibitor from the balsam pear (Momordica charantia abreviata)

AU - Vesely, David L.

AU - Graves, William R.

AU - Lo, Timothy M.

AU - Fletcher, Mary Ann

AU - Levey, Gerald S.

N1 - Funding Information: We thank Julia F. Morton who stimulated our interest in studying the balsam pear. This work was supported by NIH grant HL 13715-07 and USPHS AM-167-63-06. Dr. Levey is an Investigator of Howard Hughes Medical Institute. This work was presented at the 59th Annual Meeting of the Endocrine Society, Chicago, lllinois, June 8, 1977. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1977/8/22

Y1 - 1977/8/22

N2 - The balsam pear (Momordica charantia abreviata) is a plant which grows wild throughout subtropical regions including the Gulf Coast and Florida. The ripe fruit is highly toxic and produces mild hypoglycemia. Preparations of the ripe fruit and the leaves but not the unripe fruit or seeds inhibited guanylate cyclase activity whereas adenylate cyclase activity was unaffected. The guanylate cyclase inhibitor (GCI) has been purified about 30-fold has an estimated molecular weight of 5000 to 50,000 and is acid stable and heat labile. GCI blocked the activation of guanylate cyclase by nitroso chemical carcinogens, and therefore, may be useful in studying mechanisms of chemical carcinogenesis.

AB - The balsam pear (Momordica charantia abreviata) is a plant which grows wild throughout subtropical regions including the Gulf Coast and Florida. The ripe fruit is highly toxic and produces mild hypoglycemia. Preparations of the ripe fruit and the leaves but not the unripe fruit or seeds inhibited guanylate cyclase activity whereas adenylate cyclase activity was unaffected. The guanylate cyclase inhibitor (GCI) has been purified about 30-fold has an estimated molecular weight of 5000 to 50,000 and is acid stable and heat labile. GCI blocked the activation of guanylate cyclase by nitroso chemical carcinogens, and therefore, may be useful in studying mechanisms of chemical carcinogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0017597025&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017597025&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(77)80120-4

DO - 10.1016/S0006-291X(77)80120-4

M3 - Article

C2 - 20099

AN - SCOPUS:0017597025

VL - 77

SP - 1294

EP - 1299

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -