Isolation of α- and β-Melanocyte Stimulating Hormones (MSH) on a Preparative Scale

Andrew V. Schally; Richard N. Andersen; John M. Long; Roger Guillemin

doi:10.3181/00379727-104-25812

Isolation of α- and β-Melanocyte Stimulating Hormones (MSH) on a Preparative Scale

Andrew V. Schally, Richard N. Andersen, John M. Long, Roger Guillemin

Research output: Contribution to journal › Article

2 Scopus citations

Abstract

α- and β-MSH were isolated on preparative scale with high recovery of starting activity. The initial step in purification of both hormones is chromatography on carboxymethylcellulose. β-MSH is further purified (4-5 × 10⁶ U/mg) by use of DEAE cellulose. α-MSH was not completely freed of vasopressin and other contaminants by zone electrophoresis on large vertical column. Countercurrent distribution of concentrate of vasopressin and α-MSH, yields α-MSH with a potency of 1.5 × 10⁷ U/mg, free of vasopressin.

Original language	English (US)
Pages (from-to)	290-293
Number of pages	4
Journal	Proceedings of the Society for Experimental Biology and Medicine
Volume	104
Issue number	2
DOIs	https://doi.org/10.3181/00379727-104-25812
State	Published - Jun 1960
Externally published	Yes

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Keywords

PITUITARY GLAND/hormones

ASJC Scopus subject areas

Medicine(all)

Cite this

Schally, A. V., Andersen, R. N., Long, J. M., & Guillemin, R. (1960). Isolation of α- and β-Melanocyte Stimulating Hormones (MSH) on a Preparative Scale. Proceedings of the Society for Experimental Biology and Medicine, 104(2), 290-293. https://doi.org/10.3181/00379727-104-25812

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10.3181/00379727-104-25812