Isolation of α- and β-Melanocyte Stimulating Hormones (MSH) on a Preparative Scale

Andrew V. Schally, Richard N. Andersen, John M. Long, Roger Guillemin

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

α- and β-MSH were isolated on preparative scale with high recovery of starting activity. The initial step in purification of both hormones is chromatography on carboxymethylcellulose. β-MSH is further purified (4-5 × 106 U/mg) by use of DEAE cellulose. α-MSH was not completely freed of vasopressin and other contaminants by zone electrophoresis on large vertical column. Countercurrent distribution of concentrate of vasopressin and α-MSH, yields α-MSH with a potency of 1.5 × 107 U/mg, free of vasopressin.

Original languageEnglish (US)
Pages (from-to)290-293
Number of pages4
JournalProceedings of the Society for Experimental Biology and Medicine
Volume104
Issue number2
DOIs
StatePublished - Jun 1960

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Keywords

  • PITUITARY GLAND/hormones

ASJC Scopus subject areas

  • Medicine(all)

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