α- and β-MSH were isolated on preparative scale with high recovery of starting activity. The initial step in purification of both hormones is chromatography on carboxymethylcellulose. β-MSH is further purified (4-5 × 106 U/mg) by use of DEAE cellulose. α-MSH was not completely freed of vasopressin and other contaminants by zone electrophoresis on large vertical column. Countercurrent distribution of concentrate of vasopressin and α-MSH, yields α-MSH with a potency of 1.5 × 107 U/mg, free of vasopressin.
|Original language||English (US)|
|Number of pages||4|
|Journal||Proceedings of the Society for Experimental Biology and Medicine|
|State||Published - Jun 1960|
- PITUITARY GLAND/hormones
ASJC Scopus subject areas