Isolation of α- and β-Melanocyte Stimulating Hormones (MSH) on a Preparative Scale

Andrew V. Schally; Richard N. Andersen; John M. Long; Roger Guillemin

doi:10.3181/00379727-104-25812

Isolation of α- and β-Melanocyte Stimulating Hormones (MSH) on a Preparative Scale

Andrew V. Schally, Richard N. Andersen, John M. Long, Roger Guillemin

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

α- and β-MSH were isolated on preparative scale with high recovery of starting activity. The initial step in purification of both hormones is chromatography on carboxymethylcellulose. β-MSH is further purified (4-5 × 10⁶ U/mg) by use of DEAE cellulose. α-MSH was not completely freed of vasopressin and other contaminants by zone electrophoresis on large vertical column. Countercurrent distribution of concentrate of vasopressin and α-MSH, yields α-MSH with a potency of 1.5 × 10⁷ U/mg, free of vasopressin.

Original language	English (US)
Pages (from-to)	290-293
Number of pages	4
Journal	Proceedings of the Society for Experimental Biology and Medicine
Volume	104
Issue number	2
DOIs	https://doi.org/10.3181/00379727-104-25812
State	Published - Jun 1960
Externally published	Yes

Keywords

PITUITARY GLAND/hormones

ASJC Scopus subject areas

Medicine(all)

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10.3181/00379727-104-25812

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title = "Isolation of α- and β-Melanocyte Stimulating Hormones (MSH) on a Preparative Scale",

abstract = "α- and β-MSH were isolated on preparative scale with high recovery of starting activity. The initial step in purification of both hormones is chromatography on carboxymethylcellulose. β-MSH is further purified (4-5 × 106 U/mg) by use of DEAE cellulose. α-MSH was not completely freed of vasopressin and other contaminants by zone electrophoresis on large vertical column. Countercurrent distribution of concentrate of vasopressin and α-MSH, yields α-MSH with a potency of 1.5 × 107 U/mg, free of vasopressin.",

keywords = "PITUITARY GLAND/hormones",

author = "Schally, {Andrew V.} and Andersen, {Richard N.} and Long, {John M.} and Roger Guillemin",

note = "Funding Information: This work supported by AF contract and USPHS grant. ",

year = "1960",

month = jun,

doi = "10.3181/00379727-104-25812",

language = "English (US)",

volume = "104",

pages = "290--293",

journal = "Experimental Biology and Medicine",

issn = "0037-9727",

publisher = "Society for Experimental Biology and Medicine",

number = "2",

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TY - JOUR

T1 - Isolation of α- and β-Melanocyte Stimulating Hormones (MSH) on a Preparative Scale

AU - Schally, Andrew V.

AU - Andersen, Richard N.

AU - Long, John M.

AU - Guillemin, Roger

N1 - Funding Information: This work supported by AF contract and USPHS grant.

PY - 1960/6

Y1 - 1960/6

N2 - α- and β-MSH were isolated on preparative scale with high recovery of starting activity. The initial step in purification of both hormones is chromatography on carboxymethylcellulose. β-MSH is further purified (4-5 × 106 U/mg) by use of DEAE cellulose. α-MSH was not completely freed of vasopressin and other contaminants by zone electrophoresis on large vertical column. Countercurrent distribution of concentrate of vasopressin and α-MSH, yields α-MSH with a potency of 1.5 × 107 U/mg, free of vasopressin.

AB - α- and β-MSH were isolated on preparative scale with high recovery of starting activity. The initial step in purification of both hormones is chromatography on carboxymethylcellulose. β-MSH is further purified (4-5 × 106 U/mg) by use of DEAE cellulose. α-MSH was not completely freed of vasopressin and other contaminants by zone electrophoresis on large vertical column. Countercurrent distribution of concentrate of vasopressin and α-MSH, yields α-MSH with a potency of 1.5 × 107 U/mg, free of vasopressin.

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AN - SCOPUS:84918955376

VL - 104

SP - 290

EP - 293

JO - Experimental Biology and Medicine

JF - Experimental Biology and Medicine

SN - 0037-9727

IS - 2

ER -

Isolation of α- and β-Melanocyte Stimulating Hormones (MSH) on a Preparative Scale

Abstract

Keywords

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