Abstract
α- and β-MSH were isolated on preparative scale with high recovery of starting activity. The initial step in purification of both hormones is chromatography on carboxymethylcellulose. β-MSH is further purified (4-5 × 106 U/mg) by use of DEAE cellulose. α-MSH was not completely freed of vasopressin and other contaminants by zone electrophoresis on large vertical column. Countercurrent distribution of concentrate of vasopressin and α-MSH, yields α-MSH with a potency of 1.5 × 107 U/mg, free of vasopressin.
Original language | English (US) |
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Pages (from-to) | 290-293 |
Number of pages | 4 |
Journal | Proceedings of the Society for Experimental Biology and Medicine |
Volume | 104 |
Issue number | 2 |
DOIs | |
State | Published - Jun 1960 |
Externally published | Yes |
Keywords
- PITUITARY GLAND/hormones
ASJC Scopus subject areas
- Medicine(all)