Isolation, characterization, and expression of a cDNA encoding N- acetylglucosaminyltransferase V

M. Shoreibah, G. S. Perng, B. Adler, J. Weinstein, R. Basu, R. Cupples, D. Wen, J. K. Browne, P. Buckhaults, N. Fregien, M. Pierce

Research output: Contribution to journalArticlepeer-review

113 Scopus citations

Abstract

A cDNA clone for the complete coding sequence for α-1,3(6)-mannosylglycoprotein β-1,6-N-acetylglucosaminyltransferase V (GlcNAc-T V, EC 2.4.1.155) was isolated and expressed in COS-7 cells. Degenerate oligonucleotide primers for polymerase chain reaction were synthesized based on the amino acid sequence of three tryptic peptides isolated from affinity-purified GlcNAc-T V. Polymerase chain reaction amplimers were isolated from rat and mouse mRNA. A cDNA-encoding full-length enzyme was isolated from a rat 1 cell (EJ-ras-transformed) library and sequenced. Transient expression of this clone in COS-7 cells, followed by enzymatic activity assays, demonstrated that this cDNA sequence encodes GlcNAc-T V. Northern analysis of rat kidney mRNA revealed a single band corresponding to a length of about 7 kilobases. Sequence analysis of the cDNA clone demonstrated an open reading frame that encoded a type II membrane protein of 740 amino acids.

Original languageEnglish (US)
Pages (from-to)15381-15385
Number of pages5
JournalJournal of Biological Chemistry
Volume268
Issue number21
StatePublished - 1993

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Isolation, characterization, and expression of a cDNA encoding N- acetylglucosaminyltransferase V'. Together they form a unique fingerprint.

Cite this