Isolation and structure of somatostatin from porcine hypothalami

Andrew V Schally, Andre Dupont, Akira Arimura, Tommie W. Redding, Nozomu Nishi, George L. Linthicum, David H. Schlesinger

Research output: Contribution to journalArticle

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Abstract

The isolation and structure of somatostatin (GH-RIH) from pig hypothalami are described. This hormone was purified by preparative gel filtration, solvent extraction, countercurrent distribution in two solvent systems, ion-exchange and partition chromatography, and analytical gel filtration. The somatostatin activity was followed by in vitro bioassays and a radioimmunoassay. The isolated product was homogeneous chromatographically and had biological and immunological properties similar to synthetic somatostatin corresponding to the ovine hormone. The primary structure of porcine somatostatin was shown to be H-Ala-Gly-cyclo-(Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys)-OH. Other immunologically and biologically active form(s) of somatostatin were also detected.

Original languageEnglish
Pages (from-to)509-514
Number of pages6
JournalBiochemistry
Volume15
Issue number3
StatePublished - Dec 1 1976
Externally publishedYes

Fingerprint

Somatostatin
Hypothalamus
Swine
Gel Chromatography
Gels
Hormones
Countercurrent Distribution
Bioassay
Ion Exchange Chromatography
Solvent extraction
Chromatography
Biological Assay
Radioimmunoassay
Sheep
Ion exchange

ASJC Scopus subject areas

  • Biochemistry

Cite this

Schally, A. V., Dupont, A., Arimura, A., Redding, T. W., Nishi, N., Linthicum, G. L., & Schlesinger, D. H. (1976). Isolation and structure of somatostatin from porcine hypothalami. Biochemistry, 15(3), 509-514.

Isolation and structure of somatostatin from porcine hypothalami. / Schally, Andrew V; Dupont, Andre; Arimura, Akira; Redding, Tommie W.; Nishi, Nozomu; Linthicum, George L.; Schlesinger, David H.

In: Biochemistry, Vol. 15, No. 3, 01.12.1976, p. 509-514.

Research output: Contribution to journalArticle

Schally, AV, Dupont, A, Arimura, A, Redding, TW, Nishi, N, Linthicum, GL & Schlesinger, DH 1976, 'Isolation and structure of somatostatin from porcine hypothalami', Biochemistry, vol. 15, no. 3, pp. 509-514.
Schally AV, Dupont A, Arimura A, Redding TW, Nishi N, Linthicum GL et al. Isolation and structure of somatostatin from porcine hypothalami. Biochemistry. 1976 Dec 1;15(3):509-514.
Schally, Andrew V ; Dupont, Andre ; Arimura, Akira ; Redding, Tommie W. ; Nishi, Nozomu ; Linthicum, George L. ; Schlesinger, David H. / Isolation and structure of somatostatin from porcine hypothalami. In: Biochemistry. 1976 ; Vol. 15, No. 3. pp. 509-514.
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