Isolation and sequence of a cDNA clone for rabbit fast skeletal muscle troponin C. Homology with calmodulin and parvalbumin.

A. S. Zot, J. D. Potter, W. L. Strauss

Research output: Contribution to journalArticle

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Abstract

The binding of Ca2+ to troponin C (TnC) regulates skeletal muscle contraction. We have isolated a full-length cDNA clone for fast skeletal muscle TnC from a neonatal rabbit skeletal muscle library and determined its nucleic acid sequence. The amino acid sequence deduced from this clone matches the previously reported amino acid sequence (Collins, J. H., Greaser, M. L., Potter, J. D., and Horn, M. J. (1977) J. Biol. Chem. 252, 6356-6362) except at the amino terminus. According to the nucleotide sequence, the first 2 residues of TnC are threonine-aspartic acid, which is the reverse of the order reported previously. The isolation of the adult form of TnC from a neonatal library suggests that there may be no developmental isoforms of fast TnC. The protein coding region of the fast TnC clone has 67% homology with the reported nucleotide sequence for chicken slow TnC (Putkey, J. A., Carroll, S. L., and Means, A. R. (1987) Mol. Cell. Biol. 7, 549-1553). The homologies between the nucleotide sequences of TnC, calmodulin, and parvalbumin provide evidence that all three proteins were derived from a common precursor molecule which had four Ca2+-binding sites.

Original languageEnglish
Pages (from-to)15418-15421
Number of pages4
JournalJournal of Biological Chemistry
Volume262
Issue number32
StatePublished - Nov 15 1987
Externally publishedYes

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Troponin C
Parvalbumins
Calmodulin
Muscle
Skeletal Muscle
Complementary DNA
Clone Cells
Rabbits
Nucleotides
Amino Acid Sequence
Nucleic acid sequences
Amino Acids
Threonine
Muscle Contraction
Aspartic Acid
Nucleic Acids
Open Reading Frames
Libraries
Chickens
Protein Isoforms

ASJC Scopus subject areas

  • Biochemistry

Cite this

Isolation and sequence of a cDNA clone for rabbit fast skeletal muscle troponin C. Homology with calmodulin and parvalbumin. / Zot, A. S.; Potter, J. D.; Strauss, W. L.

In: Journal of Biological Chemistry, Vol. 262, No. 32, 15.11.1987, p. 15418-15421.

Research output: Contribution to journalArticle

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