Isolation and sequence analysis of cDNA clones coding for rat skeletal muscle creatine kinase

P. A. Benfield, R. A. Zivin, L. S. Miller, R. Sowder, G. W. Smythers, L. Henderson, S. Oroszlan, M. L. Pearson

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

A series of cDNA clones corresponding to 1494 bases of rat muscle creatine kinase mRNA has been isolated and characterized. The identify of these clones has been confirmed by DNA sequence analysis and by comparison of the predicted amino acid sequence with that determined for the purified protein. The cDNA sequence accounts for the entire coding sequence of the creatine kinase protein in addition to the complete 3' untranslated region and 68 bases of 5' noncoding region. Sequences corresponding to the active site region of the protein, the initiation codon, the termination codon, and poly(A) addition signal have been identified.

Original languageEnglish
Pages (from-to)14979-14984
Number of pages6
JournalJournal of Biological Chemistry
Volume259
Issue number23
StatePublished - Dec 1 1984

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MM Form Creatine Kinase
Sequence Analysis
Rats
Skeletal Muscle
Complementary DNA
Clone Cells
Proteins
Poly A
Initiator Codon
Terminator Codon
DNA sequences
3' Untranslated Regions
Creatine Kinase
DNA Sequence Analysis
Amino Acid Sequence
Catalytic Domain
Amino Acids
Messenger RNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Benfield, P. A., Zivin, R. A., Miller, L. S., Sowder, R., Smythers, G. W., Henderson, L., ... Pearson, M. L. (1984). Isolation and sequence analysis of cDNA clones coding for rat skeletal muscle creatine kinase. Journal of Biological Chemistry, 259(23), 14979-14984.

Isolation and sequence analysis of cDNA clones coding for rat skeletal muscle creatine kinase. / Benfield, P. A.; Zivin, R. A.; Miller, L. S.; Sowder, R.; Smythers, G. W.; Henderson, L.; Oroszlan, S.; Pearson, M. L.

In: Journal of Biological Chemistry, Vol. 259, No. 23, 01.12.1984, p. 14979-14984.

Research output: Contribution to journalArticle

Benfield, PA, Zivin, RA, Miller, LS, Sowder, R, Smythers, GW, Henderson, L, Oroszlan, S & Pearson, ML 1984, 'Isolation and sequence analysis of cDNA clones coding for rat skeletal muscle creatine kinase', Journal of Biological Chemistry, vol. 259, no. 23, pp. 14979-14984.
Benfield PA, Zivin RA, Miller LS, Sowder R, Smythers GW, Henderson L et al. Isolation and sequence analysis of cDNA clones coding for rat skeletal muscle creatine kinase. Journal of Biological Chemistry. 1984 Dec 1;259(23):14979-14984.
Benfield, P. A. ; Zivin, R. A. ; Miller, L. S. ; Sowder, R. ; Smythers, G. W. ; Henderson, L. ; Oroszlan, S. ; Pearson, M. L. / Isolation and sequence analysis of cDNA clones coding for rat skeletal muscle creatine kinase. In: Journal of Biological Chemistry. 1984 ; Vol. 259, No. 23. pp. 14979-14984.
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