Isolation and chromosomal localization of a new human retinoblastoma binding protein 2 homologue 1a (RBBP2H1A)

Vladimir Kashuba, Alexei Protopopov, Raf Podowski, Rinat Gizatullin, Jingfeng Li, George Klein, Claes Wahlestedt, Eugene Zabarovsky

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Using a NotI linking clone NR-025 as a probe, we isolated a novel putative member of the RB binding protein family, namely a human retinoblastoma binding protein 2 homologue (RBBP2H1A). The maximal open reading frame encodes a protein of 1681 amino acids. Homology analysis indicated that the predicted product has an overall 56% amino acid identity to RBBP2, which plays an important role in RE tumor suppressor regulation. Many extended regions are 100% identical in amino acids sequences. The degree of nucleotide identity is lower. The structure prediction analysis identified three DNA-binding zinc finger domains and two bipartite nuclear localization signals. Northern expression analysis revealed expression in all tissues; however, the level of expression significantly varied between tissues. The highest level of expression was detected in testis and the lowest in skeletal muscle. The mRNA sizes corresponding to two major products are around 6 kb and 7 kb. Using fluorescence in situ hybridization, we mapped the gene to chromosomal band 1q32.1.

Original languageEnglish (US)
Pages (from-to)407-413
Number of pages7
JournalEuropean Journal of Human Genetics
Volume8
Issue number6
DOIs
StatePublished - Jun 1 2000
Externally publishedYes

Keywords

  • Fluorescence in situ hybridization
  • Gene mapping
  • Human
  • Molecular cloning
  • NotI-linking clone
  • Sequence alignment

ASJC Scopus subject areas

  • Genetics(clinical)

Fingerprint Dive into the research topics of 'Isolation and chromosomal localization of a new human retinoblastoma binding protein 2 homologue 1a (RBBP2H1A)'. Together they form a unique fingerprint.

  • Cite this