Isolation and characterization of xnov, a Xenopus laevis ortholog of the chicken nov gene

Zhentu Ying, Mary Lou L King

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We have isolated an ortholog (xnov) of the chicken nov gene (for nephroblastoma overexpressed; encoding a putative avian proto-oncogene) from Xenopus laevis (Xl) by screening an Xl ovary cDNA library and genomic library using the entire coding region of human CTGF (encoding connective tissue growth factor) as a probe and by 5'RACE (rapid amplification of cDNA ends), xnov has the same genomic organization as chicken nov, mouse fisp12 and cyr61, but has a unique promoter sequence. The Xl open reading frame (ORF) encodes a 343-amino-acid (aa) polypeptide of 37.9 kDa, Xnov shows 62.9, 60.5, 52.2, 52.1, 47.6 and 45.8% identity with the chicken Nov, human NovH, human CTGF, mouse Fisp12, chicken Cef10 and mouse Cyr61 proteins, respectively. Xnov contains four aa domains which characterize the CTGF family, RT-PCR (reverse transcription-polymerase chain reaction) analysis shows that the xnov mRNA is very low in abundance and appears to be present throughout early XI development. Our results also indicate that xnov and nov are not orthologs of human CTGF.

Original languageEnglish
Pages (from-to)243-248
Number of pages6
JournalGene
Volume171
Issue number2
DOIs
StatePublished - Jun 1 1996

Fingerprint

Xenopus laevis
Chickens
Genes
Connective Tissue Growth Factor
Amino Acids
Wilms Tumor
Proto-Oncogenes
Genomic Library
Gene Library
Open Reading Frames
Reverse Transcription
Ovary
Complementary DNA
Polymerase Chain Reaction
Messenger RNA
Peptides
human CTGF protein

Keywords

  • Connective tissue growth factor family
  • Expression in frog development
  • Genomic organization
  • Xnov promoter

ASJC Scopus subject areas

  • Genetics

Cite this

Isolation and characterization of xnov, a Xenopus laevis ortholog of the chicken nov gene. / Ying, Zhentu; King, Mary Lou L.

In: Gene, Vol. 171, No. 2, 01.06.1996, p. 243-248.

Research output: Contribution to journalArticle

Ying, Zhentu ; King, Mary Lou L. / Isolation and characterization of xnov, a Xenopus laevis ortholog of the chicken nov gene. In: Gene. 1996 ; Vol. 171, No. 2. pp. 243-248.
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