TY - JOUR
T1 - Isolation and characterization of xnov, a Xenopus laevis ortholog of the chicken nov gene
AU - Ying, Zhentu
AU - King, Mary Lou
N1 - Funding Information:
We would like to thank Drs. Martin Flajnik (University of Miami, Miami, FL), for giving us a XI genomic library, Doug Melton (Harvard University, Cambridge, MA) for providing us with a XI ovary cDNA library and finally, Robert Tompkins (Tulane University, New Orleans, LA) for sending us XI thymic tumor cells. We would especially like to acknowledge Dr. Gary Grotendorst for his generous gift of human CTGF cDNA as well as his helpful advice. We thank Jian Zhang, Yi Zhou, Heather MacArthur, Julia Saludes and Dr. Kai Wu for their useful suggestions throughout the course of these studies. This work was supported by National Institutes of Health Grant #GM33932 to M.L.K..
PY - 1996/6/1
Y1 - 1996/6/1
N2 - We have isolated an ortholog (xnov) of the chicken nov gene (for nephroblastoma overexpressed; encoding a putative avian proto-oncogene) from Xenopus laevis (Xl) by screening an Xl ovary cDNA library and genomic library using the entire coding region of human CTGF (encoding connective tissue growth factor) as a probe and by 5'RACE (rapid amplification of cDNA ends), xnov has the same genomic organization as chicken nov, mouse fisp12 and cyr61, but has a unique promoter sequence. The Xl open reading frame (ORF) encodes a 343-amino-acid (aa) polypeptide of 37.9 kDa, Xnov shows 62.9, 60.5, 52.2, 52.1, 47.6 and 45.8% identity with the chicken Nov, human NovH, human CTGF, mouse Fisp12, chicken Cef10 and mouse Cyr61 proteins, respectively. Xnov contains four aa domains which characterize the CTGF family, RT-PCR (reverse transcription-polymerase chain reaction) analysis shows that the xnov mRNA is very low in abundance and appears to be present throughout early XI development. Our results also indicate that xnov and nov are not orthologs of human CTGF.
AB - We have isolated an ortholog (xnov) of the chicken nov gene (for nephroblastoma overexpressed; encoding a putative avian proto-oncogene) from Xenopus laevis (Xl) by screening an Xl ovary cDNA library and genomic library using the entire coding region of human CTGF (encoding connective tissue growth factor) as a probe and by 5'RACE (rapid amplification of cDNA ends), xnov has the same genomic organization as chicken nov, mouse fisp12 and cyr61, but has a unique promoter sequence. The Xl open reading frame (ORF) encodes a 343-amino-acid (aa) polypeptide of 37.9 kDa, Xnov shows 62.9, 60.5, 52.2, 52.1, 47.6 and 45.8% identity with the chicken Nov, human NovH, human CTGF, mouse Fisp12, chicken Cef10 and mouse Cyr61 proteins, respectively. Xnov contains four aa domains which characterize the CTGF family, RT-PCR (reverse transcription-polymerase chain reaction) analysis shows that the xnov mRNA is very low in abundance and appears to be present throughout early XI development. Our results also indicate that xnov and nov are not orthologs of human CTGF.
KW - Connective tissue growth factor family
KW - Expression in frog development
KW - Genomic organization
KW - Xnov promoter
UR - http://www.scopus.com/inward/record.url?scp=0029945768&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029945768&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(95)00891-8
DO - 10.1016/0378-1119(95)00891-8
M3 - Article
C2 - 8666280
AN - SCOPUS:0029945768
VL - 171
SP - 243
EP - 248
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -