Isolation and Characterization of Monoclonal Antibodies Specific for the Cardiac Muscarinic Acetylcholine Receptor

Charles W. Luetje, Craig Brumwell, Marjorie Gainer Norman, Neil M. Nathanson, Gary L. Peterson, Michael I. Schimerlik

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

A number of monoclonal antibodies were raised against the purified porcine atrial muscarinic acetylcholine receptor. The antibodies were shown to exhibit a high degree of specificity for the receptor by their ability to recognize the purified receptor but not other porcine atrial glycoproteins in enzyme-linked solid-phase immunosorptive assays and by immunoblot analyses. Several of the antibodies were able to quantitatively precipitate the muscarinic receptor in both pig and rat heart and a portion of the receptor from rat cerebellum but little if any receptor from rat cerebral cortex. Thus, these monoclonal antibodies not only exhibit specificity for the muscarinic receptor but also are specific for the cardiac receptor subtype.

Original languageEnglish (US)
Pages (from-to)6892-6896
Number of pages5
JournalBiochemistry
Volume26
Issue number22
DOIs
StatePublished - 1987

ASJC Scopus subject areas

  • Biochemistry

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    Luetje, C. W., Brumwell, C., Norman, M. G., Nathanson, N. M., Peterson, G. L., & Schimerlik, M. I. (1987). Isolation and Characterization of Monoclonal Antibodies Specific for the Cardiac Muscarinic Acetylcholine Receptor. Biochemistry, 26(22), 6892-6896. https://doi.org/10.1021/bi00396a003