Abstract
Genetic variation has been difficult to detect in striped bass (Morone saxatilis). Therefore, we identified and characterized 13 microsatellite loci to provide additional genetic markers for striped bass. Microsatellites were identified by screening a striped bass genomic library or by using primers developed for European sea bass (Dicentrarchus labrax) microsatellite loci. We found that 6 of the 13 microsatellite loci were polymorphic in DNA samples obtained from wild populations of striped bass. The number of alleles per locus varied from 3 to 12, and the observed heterozygosities ranged from 0.55 to 0.78. These results indicate that microsatellite loci provide more alleles and higher heterozygosities than other genetic markers developed for striped bass.
| Original language | English |
|---|---|
| Pages (from-to) | 405-408 |
| Number of pages | 4 |
| Journal | Marine Biotechnology |
| Volume | 2 |
| Issue number | 5 |
| State | Published - Dec 6 2000 |
| Externally published | Yes |
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Keywords
- Microsatellites
- Morone saxatilis
- Polymorphism
- Striped bass
ASJC Scopus subject areas
- Aquatic Science
- Biotechnology
Cite this
Isolation and characterization of microsatellite loci for striped bass (Morone saxatilis). / Han, Kaiping; Li, Li; Leclerc, Gilles M.; Hays, Alison M.; Ely, Bert.
In: Marine Biotechnology, Vol. 2, No. 5, 06.12.2000, p. 405-408.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation and characterization of microsatellite loci for striped bass (Morone saxatilis)
AU - Han, Kaiping
AU - Li, Li
AU - Leclerc, Gilles M.
AU - Hays, Alison M.
AU - Ely, Bert
PY - 2000/12/6
Y1 - 2000/12/6
N2 - Genetic variation has been difficult to detect in striped bass (Morone saxatilis). Therefore, we identified and characterized 13 microsatellite loci to provide additional genetic markers for striped bass. Microsatellites were identified by screening a striped bass genomic library or by using primers developed for European sea bass (Dicentrarchus labrax) microsatellite loci. We found that 6 of the 13 microsatellite loci were polymorphic in DNA samples obtained from wild populations of striped bass. The number of alleles per locus varied from 3 to 12, and the observed heterozygosities ranged from 0.55 to 0.78. These results indicate that microsatellite loci provide more alleles and higher heterozygosities than other genetic markers developed for striped bass.
AB - Genetic variation has been difficult to detect in striped bass (Morone saxatilis). Therefore, we identified and characterized 13 microsatellite loci to provide additional genetic markers for striped bass. Microsatellites were identified by screening a striped bass genomic library or by using primers developed for European sea bass (Dicentrarchus labrax) microsatellite loci. We found that 6 of the 13 microsatellite loci were polymorphic in DNA samples obtained from wild populations of striped bass. The number of alleles per locus varied from 3 to 12, and the observed heterozygosities ranged from 0.55 to 0.78. These results indicate that microsatellite loci provide more alleles and higher heterozygosities than other genetic markers developed for striped bass.
KW - Microsatellites
KW - Morone saxatilis
KW - Polymorphism
KW - Striped bass
UR - http://www.scopus.com/inward/record.url?scp=0033670644&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033670644&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0033670644
VL - 2
SP - 405
EP - 408
JO - Marine Biotechnology
JF - Marine Biotechnology
SN - 1436-2228
IS - 5
ER -