Isolation and characterization of microsatellite loci for striped bass (Morone saxatilis)

Kaiping Han, Li Li, Gilles M. Leclerc, Alison M. Hays, Bert Ely

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Genetic variation has been difficult to detect in striped bass (Morone saxatilis). Therefore, we identified and characterized 13 microsatellite loci to provide additional genetic markers for striped bass. Microsatellites were identified by screening a striped bass genomic library or by using primers developed for European sea bass (Dicentrarchus labrax) microsatellite loci. We found that 6 of the 13 microsatellite loci were polymorphic in DNA samples obtained from wild populations of striped bass. The number of alleles per locus varied from 3 to 12, and the observed heterozygosities ranged from 0.55 to 0.78. These results indicate that microsatellite loci provide more alleles and higher heterozygosities than other genetic markers developed for striped bass.

Original languageEnglish
Pages (from-to)405-408
Number of pages4
JournalMarine Biotechnology
Volume2
Issue number5
StatePublished - Dec 6 2000
Externally publishedYes

Fingerprint

Bass
Morone saxatilis
genetic marker
heterozygosity
Microsatellite Repeats
allele
microsatellite repeats
loci
wild population
genetic variation
genomics
Dicentrarchus labrax
DNA
Genetic Markers
alleles
genetic markers
Alleles
genomic libraries
Genomic Library
screening

Keywords

  • Microsatellites
  • Morone saxatilis
  • Polymorphism
  • Striped bass

ASJC Scopus subject areas

  • Aquatic Science
  • Biotechnology

Cite this

Han, K., Li, L., Leclerc, G. M., Hays, A. M., & Ely, B. (2000). Isolation and characterization of microsatellite loci for striped bass (Morone saxatilis). Marine Biotechnology, 2(5), 405-408.

Isolation and characterization of microsatellite loci for striped bass (Morone saxatilis). / Han, Kaiping; Li, Li; Leclerc, Gilles M.; Hays, Alison M.; Ely, Bert.

In: Marine Biotechnology, Vol. 2, No. 5, 06.12.2000, p. 405-408.

Research output: Contribution to journalArticle

Han, K, Li, L, Leclerc, GM, Hays, AM & Ely, B 2000, 'Isolation and characterization of microsatellite loci for striped bass (Morone saxatilis)', Marine Biotechnology, vol. 2, no. 5, pp. 405-408.
Han, Kaiping ; Li, Li ; Leclerc, Gilles M. ; Hays, Alison M. ; Ely, Bert. / Isolation and characterization of microsatellite loci for striped bass (Morone saxatilis). In: Marine Biotechnology. 2000 ; Vol. 2, No. 5. pp. 405-408.
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