Isolation and characterization of microsatellite loci for striped bass (Morone saxatilis)

Kaiping Han; Li Li; Gilles M. Leclerc; Alison M. Hays; Bert Ely

doi:10.1007/s101260000014

Isolation and characterization of microsatellite loci for striped bass (Morone saxatilis)

Kaiping Han, Li Li, Gilles M. Leclerc, Alison M. Hays, Bert Ely

Pediatrics

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16 Scopus citations

Abstract

Genetic variation has been difficult to detect in striped bass (Morone saxatilis). Therefore, we identified and characterized 13 microsatellite loci to provide additional genetic markers for striped bass. Microsatellites were identified by screening a striped bass genomic library or by using primers developed for European sea bass (Dicentrarchus labrax) microsatellite loci. We found that 6 of the 13 microsatellite loci were polymorphic in DNA samples obtained from wild populations of striped bass. The number of alleles per locus varied from 3 to 12, and the observed heterozygosities ranged from 0.55 to 0.78. These results indicate that microsatellite loci provide more alleles and higher heterozygosities than other genetic markers developed for striped bass.

Original language	English (US)
Pages (from-to)	405-408
Number of pages	4
Journal	Marine Biotechnology
Volume	2
Issue number	5
DOIs	https://doi.org/10.1007/s101260000014
State	Published - 2000

Keywords

Microsatellites
Morone saxatilis
Polymorphism
Striped bass

ASJC Scopus subject areas

Aquatic Science
Biotechnology

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10.1007/s101260000014

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title = "Isolation and characterization of microsatellite loci for striped bass (Morone saxatilis)",

abstract = "Genetic variation has been difficult to detect in striped bass (Morone saxatilis). Therefore, we identified and characterized 13 microsatellite loci to provide additional genetic markers for striped bass. Microsatellites were identified by screening a striped bass genomic library or by using primers developed for European sea bass (Dicentrarchus labrax) microsatellite loci. We found that 6 of the 13 microsatellite loci were polymorphic in DNA samples obtained from wild populations of striped bass. The number of alleles per locus varied from 3 to 12, and the observed heterozygosities ranged from 0.55 to 0.78. These results indicate that microsatellite loci provide more alleles and higher heterozygosities than other genetic markers developed for striped bass.",

keywords = "Microsatellites, Morone saxatilis, Polymorphism, Striped bass",

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year = "2000",

doi = "10.1007/s101260000014",

language = "English (US)",

volume = "2",

pages = "405--408",

journal = "Molecular Marine Biology and Biotechnology",

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TY - JOUR

T1 - Isolation and characterization of microsatellite loci for striped bass (Morone saxatilis)

AU - Han, Kaiping

AU - Li, Li

AU - Leclerc, Gilles M.

AU - Hays, Alison M.

AU - Ely, Bert

PY - 2000

Y1 - 2000

N2 - Genetic variation has been difficult to detect in striped bass (Morone saxatilis). Therefore, we identified and characterized 13 microsatellite loci to provide additional genetic markers for striped bass. Microsatellites were identified by screening a striped bass genomic library or by using primers developed for European sea bass (Dicentrarchus labrax) microsatellite loci. We found that 6 of the 13 microsatellite loci were polymorphic in DNA samples obtained from wild populations of striped bass. The number of alleles per locus varied from 3 to 12, and the observed heterozygosities ranged from 0.55 to 0.78. These results indicate that microsatellite loci provide more alleles and higher heterozygosities than other genetic markers developed for striped bass.

AB - Genetic variation has been difficult to detect in striped bass (Morone saxatilis). Therefore, we identified and characterized 13 microsatellite loci to provide additional genetic markers for striped bass. Microsatellites were identified by screening a striped bass genomic library or by using primers developed for European sea bass (Dicentrarchus labrax) microsatellite loci. We found that 6 of the 13 microsatellite loci were polymorphic in DNA samples obtained from wild populations of striped bass. The number of alleles per locus varied from 3 to 12, and the observed heterozygosities ranged from 0.55 to 0.78. These results indicate that microsatellite loci provide more alleles and higher heterozygosities than other genetic markers developed for striped bass.

KW - Microsatellites

KW - Morone saxatilis

KW - Polymorphism

KW - Striped bass

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Isolation and characterization of microsatellite loci for striped bass (Morone saxatilis)

Abstract

Keywords

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