Isolation and characterization of a tamoxifen-resistant cell line derived from MCF-7 human breast cancer cells

H. Nawata, D. Bronzert, Marc E Lippman

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

A tamoxifen-resistant variant (R27) was selected by cloning wild type MCF-7 cells. R27 has the same growth rate as MCF-7 under optimal conditions. But tamoxifen has no effect on the growth rate (growth constant of R27 under control condition, 0.071±0.007 day-1; tamoxifen-treated cells, 0.073±0.011 day-1) and minimal effects on thymidine incorporation in R27, whereas tamoxifen has a strongly suppressive effect on the growth rate and thymidine incorporation in MCF-7. The estradiol-mediated stimulation of growth in R27 is somewhat less than in MCF-7 (growth constants of R27 and MCF-7 are 0.112±0.015 and 0.125±0.009 day-1, respectively. R27 contains unoccupied receptors in both cytoplasmic and crude nuclear extract fractions with slightly higher numbers of cytosol receptors than wild type MCF-7. The same dissociation constant and the same molecular weight estimated by Sephadex chromatography and the same sucrose density behavior were observed in R27 and MCF-7. Extent of competition with estradiol and tamoxifen for cytoplasmic estrogen receptor is the same for R27 and MCF-7. Induction of progesterone receptor following treatment with estradiol is the same for R27 and MCF-7. Estrogen receptor is activated by salt and nucleotide in both MCF-7 and R27; however, the extent of activation is much higher in MCF-7 than R27.

Original languageEnglish
Pages (from-to)5016-5021
Number of pages6
JournalJournal of Biological Chemistry
Volume256
Issue number10
StatePublished - Dec 1 1981
Externally publishedYes

Fingerprint

Tamoxifen
Cells
Breast Neoplasms
Cell Line
Growth
Estradiol
Estrogen Receptors
Thymidine
Cloning
Progesterone Receptors
Chromatography
MCF-7 Cells
Sucrose
Cytoplasmic and Nuclear Receptors
Complex Mixtures
Cytosol
Nucleotides
Salts
Chemical activation
Molecular weight

ASJC Scopus subject areas

  • Biochemistry

Cite this

Isolation and characterization of a tamoxifen-resistant cell line derived from MCF-7 human breast cancer cells. / Nawata, H.; Bronzert, D.; Lippman, Marc E.

In: Journal of Biological Chemistry, Vol. 256, No. 10, 01.12.1981, p. 5016-5021.

Research output: Contribution to journalArticle

Nawata, H. ; Bronzert, D. ; Lippman, Marc E. / Isolation and characterization of a tamoxifen-resistant cell line derived from MCF-7 human breast cancer cells. In: Journal of Biological Chemistry. 1981 ; Vol. 256, No. 10. pp. 5016-5021.
@article{10ae2e3e7af74a01bf58c89f7923b48e,
title = "Isolation and characterization of a tamoxifen-resistant cell line derived from MCF-7 human breast cancer cells",
abstract = "A tamoxifen-resistant variant (R27) was selected by cloning wild type MCF-7 cells. R27 has the same growth rate as MCF-7 under optimal conditions. But tamoxifen has no effect on the growth rate (growth constant of R27 under control condition, 0.071±0.007 day-1; tamoxifen-treated cells, 0.073±0.011 day-1) and minimal effects on thymidine incorporation in R27, whereas tamoxifen has a strongly suppressive effect on the growth rate and thymidine incorporation in MCF-7. The estradiol-mediated stimulation of growth in R27 is somewhat less than in MCF-7 (growth constants of R27 and MCF-7 are 0.112±0.015 and 0.125±0.009 day-1, respectively. R27 contains unoccupied receptors in both cytoplasmic and crude nuclear extract fractions with slightly higher numbers of cytosol receptors than wild type MCF-7. The same dissociation constant and the same molecular weight estimated by Sephadex chromatography and the same sucrose density behavior were observed in R27 and MCF-7. Extent of competition with estradiol and tamoxifen for cytoplasmic estrogen receptor is the same for R27 and MCF-7. Induction of progesterone receptor following treatment with estradiol is the same for R27 and MCF-7. Estrogen receptor is activated by salt and nucleotide in both MCF-7 and R27; however, the extent of activation is much higher in MCF-7 than R27.",
author = "H. Nawata and D. Bronzert and Lippman, {Marc E}",
year = "1981",
month = "12",
day = "1",
language = "English",
volume = "256",
pages = "5016--5021",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "10",

}

TY - JOUR

T1 - Isolation and characterization of a tamoxifen-resistant cell line derived from MCF-7 human breast cancer cells

AU - Nawata, H.

AU - Bronzert, D.

AU - Lippman, Marc E

PY - 1981/12/1

Y1 - 1981/12/1

N2 - A tamoxifen-resistant variant (R27) was selected by cloning wild type MCF-7 cells. R27 has the same growth rate as MCF-7 under optimal conditions. But tamoxifen has no effect on the growth rate (growth constant of R27 under control condition, 0.071±0.007 day-1; tamoxifen-treated cells, 0.073±0.011 day-1) and minimal effects on thymidine incorporation in R27, whereas tamoxifen has a strongly suppressive effect on the growth rate and thymidine incorporation in MCF-7. The estradiol-mediated stimulation of growth in R27 is somewhat less than in MCF-7 (growth constants of R27 and MCF-7 are 0.112±0.015 and 0.125±0.009 day-1, respectively. R27 contains unoccupied receptors in both cytoplasmic and crude nuclear extract fractions with slightly higher numbers of cytosol receptors than wild type MCF-7. The same dissociation constant and the same molecular weight estimated by Sephadex chromatography and the same sucrose density behavior were observed in R27 and MCF-7. Extent of competition with estradiol and tamoxifen for cytoplasmic estrogen receptor is the same for R27 and MCF-7. Induction of progesterone receptor following treatment with estradiol is the same for R27 and MCF-7. Estrogen receptor is activated by salt and nucleotide in both MCF-7 and R27; however, the extent of activation is much higher in MCF-7 than R27.

AB - A tamoxifen-resistant variant (R27) was selected by cloning wild type MCF-7 cells. R27 has the same growth rate as MCF-7 under optimal conditions. But tamoxifen has no effect on the growth rate (growth constant of R27 under control condition, 0.071±0.007 day-1; tamoxifen-treated cells, 0.073±0.011 day-1) and minimal effects on thymidine incorporation in R27, whereas tamoxifen has a strongly suppressive effect on the growth rate and thymidine incorporation in MCF-7. The estradiol-mediated stimulation of growth in R27 is somewhat less than in MCF-7 (growth constants of R27 and MCF-7 are 0.112±0.015 and 0.125±0.009 day-1, respectively. R27 contains unoccupied receptors in both cytoplasmic and crude nuclear extract fractions with slightly higher numbers of cytosol receptors than wild type MCF-7. The same dissociation constant and the same molecular weight estimated by Sephadex chromatography and the same sucrose density behavior were observed in R27 and MCF-7. Extent of competition with estradiol and tamoxifen for cytoplasmic estrogen receptor is the same for R27 and MCF-7. Induction of progesterone receptor following treatment with estradiol is the same for R27 and MCF-7. Estrogen receptor is activated by salt and nucleotide in both MCF-7 and R27; however, the extent of activation is much higher in MCF-7 than R27.

UR - http://www.scopus.com/inward/record.url?scp=0019797751&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019797751&partnerID=8YFLogxK

M3 - Article

VL - 256

SP - 5016

EP - 5021

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 10

ER -