Isolation and Characterization of a Peroxidase from the Airway

Matthias A Salathe, Marisol Holderby, Rosanna Forteza, William M. Abraham, Adam Wanner, Gregory E. Conner

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Sheep airway mucus can potently scavenge hydrogen peroxide, an important mediator of airway inflammation. Here, the scavenging activity was identified as a peroxidase produced by goblet cells of the airway epithelium and secreted into the airway lumen. Ovine airway peroxidase activity was purified ∼ 100-fold from airway lavage fluid in two steps, using cation exchange and lectin affinity chromatography, yielding an apparently homogeneous 82-kD glycoprotein. Ovine airway peroxidase represented about 1% of the total protein in airway mucus and thus was an abundant enzyme in airway secretions. The absorbance spectrum of the purified peroxidase showed a major peak at 412 nm indicative of a hemoprotein. The ratio of A412/A280 of the purified enzyme was 0.86. The absorption spectrum of ovine airway peroxidase, its ability to oxidize halides, its sensitivity to inhibitors and its apparent molecular mass on sodium dodecyl sulfate gels showed that airway peroxidase was similar to lactoperoxidase but distinguished from myeloperoxidase, eosinophil peroxidase as well as from glutathione peroxidases. Based on these observations, ovine airway peroxidase is a newly isolated and abundant enzyme of airway mucus which may function to control reactive oxygen species in the airway and to prevent infection by catalyzing the formation of biocidal compounds.

Original languageEnglish
Pages (from-to)97-105
Number of pages9
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume17
Issue number1
StatePublished - Dec 1 1997

Fingerprint

Peroxidase
Sheep
Mucus
Enzymes
Eosinophil Peroxidase
Lactoperoxidase
Inflammation Mediators
Goblet Cells
Therapeutic Irrigation
Glutathione Peroxidase
Affinity Chromatography
Lectins
Sodium Dodecyl Sulfate
Hydrogen Peroxide
Cations
Reactive Oxygen Species
Glycoproteins
Epithelium
Gels
Affinity chromatography

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Pulmonary and Respiratory Medicine

Cite this

Isolation and Characterization of a Peroxidase from the Airway. / Salathe, Matthias A; Holderby, Marisol; Forteza, Rosanna; Abraham, William M.; Wanner, Adam; Conner, Gregory E.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 17, No. 1, 01.12.1997, p. 97-105.

Research output: Contribution to journalArticle

Salathe, Matthias A ; Holderby, Marisol ; Forteza, Rosanna ; Abraham, William M. ; Wanner, Adam ; Conner, Gregory E. / Isolation and Characterization of a Peroxidase from the Airway. In: American Journal of Respiratory Cell and Molecular Biology. 1997 ; Vol. 17, No. 1. pp. 97-105.
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