Isolation and characterization of 20 polymorphic microsatellite loci for Scaptodrosophila hibisci

Alexandra Wilson, Paul Sunnucks, J. S F Barker

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Scaptodrosophila hibisci is an endemic Australian Drosophilidae that breeds in the flowers of native Hibiscus. Here we report the isolation and amplification of 20 polymorphic microsatellite loci. We cloned these microsatellites because loci developed for Drosophila melanogaster failed to amplify in S. hibisci. Null alleles were detected at six loci, and five were X-linked. Two of the primer pairs amplified an unlinked 'bonus' locus. One locus containing juxtaposed microsatellite loci was suitable for designing an additional set of primers. Mean number of alleles per locus was 10, mean HO and HE per locus were 0.532 and 0.636, respectively.

Original languageEnglish
Pages (from-to)242-244
Number of pages3
JournalMolecular Ecology Notes
Volume2
Issue number3
DOIs
StatePublished - Sep 1 2002
Externally publishedYes

Fingerprint

Microsatellite Repeats
allele
microsatellite repeats
loci
Drosophilidae
Alleles
Hibiscus
amplification
flower
Drosophila melanogaster
Amplification
null alleles
alleles
breeds
flowers

Keywords

  • Drosophilidae
  • Juxtaposed loci
  • Null alleles
  • Segregation distortion
  • Sex linkage
  • X-linkage

ASJC Scopus subject areas

  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Isolation and characterization of 20 polymorphic microsatellite loci for Scaptodrosophila hibisci. / Wilson, Alexandra; Sunnucks, Paul; Barker, J. S F.

In: Molecular Ecology Notes, Vol. 2, No. 3, 01.09.2002, p. 242-244.

Research output: Contribution to journalArticle

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