Isolating aging mutants: A novel method yields three strains of the nematode Caenorhabditis elegans with extended life spans

Yulong Yang, David L. Wilson

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We designed a novel procedure for the isolation of mutant strains with significantly increased life spans in the nematode Caenorhabditis elegans. This procedure involves using heat-shock to screen a large number of animals and isolate a few which are more resistant to heat-shock stress. From the heat-shock-resistant animals, three mutant strains, HG25, HG96, and HG246, all exhibiting increased life span, were isolated. One mutant strain (HG246) develops more slowly than the wild-type strain, N2. Two mutant strains, HG96 and HG246, exhibit lower fertility than the wild-type. Each of the three mutant strains has a normal appearance. Their locomotive behavior also appears normal; only HG246 shows slightly slower movement. Their feeding behavior appears normal, and the males of HG25 and HG96 show normal mating behavior. However, the males of HG246, either are defective in their mating ability or their sperm are defective. The results indicate that heat-shock can be used as a means to facilitate the isolation of mutants which have longer life expectancy. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)101-116
Number of pages16
JournalMechanisms of Ageing and Development
Volume113
Issue number2
DOIs
StatePublished - Feb 7 2000

Fingerprint

Caenorhabditis elegans
Shock
Hot Temperature
Aging of materials
Animals
Aptitude
Locomotives
Feeding Behavior
Life Expectancy
Fertility
Spermatozoa

Keywords

  • Aging
  • C. elegans
  • Heat-shock
  • Life span
  • Longevity
  • Mutant

ASJC Scopus subject areas

  • Aging
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

Cite this

Isolating aging mutants : A novel method yields three strains of the nematode Caenorhabditis elegans with extended life spans. / Yang, Yulong; Wilson, David L.

In: Mechanisms of Ageing and Development, Vol. 113, No. 2, 07.02.2000, p. 101-116.

Research output: Contribution to journalArticle

@article{a8609230ccfa497b9a65021cc17a4421,
title = "Isolating aging mutants: A novel method yields three strains of the nematode Caenorhabditis elegans with extended life spans",
abstract = "We designed a novel procedure for the isolation of mutant strains with significantly increased life spans in the nematode Caenorhabditis elegans. This procedure involves using heat-shock to screen a large number of animals and isolate a few which are more resistant to heat-shock stress. From the heat-shock-resistant animals, three mutant strains, HG25, HG96, and HG246, all exhibiting increased life span, were isolated. One mutant strain (HG246) develops more slowly than the wild-type strain, N2. Two mutant strains, HG96 and HG246, exhibit lower fertility than the wild-type. Each of the three mutant strains has a normal appearance. Their locomotive behavior also appears normal; only HG246 shows slightly slower movement. Their feeding behavior appears normal, and the males of HG25 and HG96 show normal mating behavior. However, the males of HG246, either are defective in their mating ability or their sperm are defective. The results indicate that heat-shock can be used as a means to facilitate the isolation of mutants which have longer life expectancy. Copyright (C) 2000 Elsevier Science Ireland Ltd.",
keywords = "Aging, C. elegans, Heat-shock, Life span, Longevity, Mutant",
author = "Yulong Yang and Wilson, {David L.}",
year = "2000",
month = "2",
day = "7",
doi = "10.1016/S0047-6374(99)00100-1",
language = "English",
volume = "113",
pages = "101--116",
journal = "Mechanisms of Ageing and Development",
issn = "0047-6374",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Isolating aging mutants

T2 - A novel method yields three strains of the nematode Caenorhabditis elegans with extended life spans

AU - Yang, Yulong

AU - Wilson, David L.

PY - 2000/2/7

Y1 - 2000/2/7

N2 - We designed a novel procedure for the isolation of mutant strains with significantly increased life spans in the nematode Caenorhabditis elegans. This procedure involves using heat-shock to screen a large number of animals and isolate a few which are more resistant to heat-shock stress. From the heat-shock-resistant animals, three mutant strains, HG25, HG96, and HG246, all exhibiting increased life span, were isolated. One mutant strain (HG246) develops more slowly than the wild-type strain, N2. Two mutant strains, HG96 and HG246, exhibit lower fertility than the wild-type. Each of the three mutant strains has a normal appearance. Their locomotive behavior also appears normal; only HG246 shows slightly slower movement. Their feeding behavior appears normal, and the males of HG25 and HG96 show normal mating behavior. However, the males of HG246, either are defective in their mating ability or their sperm are defective. The results indicate that heat-shock can be used as a means to facilitate the isolation of mutants which have longer life expectancy. Copyright (C) 2000 Elsevier Science Ireland Ltd.

AB - We designed a novel procedure for the isolation of mutant strains with significantly increased life spans in the nematode Caenorhabditis elegans. This procedure involves using heat-shock to screen a large number of animals and isolate a few which are more resistant to heat-shock stress. From the heat-shock-resistant animals, three mutant strains, HG25, HG96, and HG246, all exhibiting increased life span, were isolated. One mutant strain (HG246) develops more slowly than the wild-type strain, N2. Two mutant strains, HG96 and HG246, exhibit lower fertility than the wild-type. Each of the three mutant strains has a normal appearance. Their locomotive behavior also appears normal; only HG246 shows slightly slower movement. Their feeding behavior appears normal, and the males of HG25 and HG96 show normal mating behavior. However, the males of HG246, either are defective in their mating ability or their sperm are defective. The results indicate that heat-shock can be used as a means to facilitate the isolation of mutants which have longer life expectancy. Copyright (C) 2000 Elsevier Science Ireland Ltd.

KW - Aging

KW - C. elegans

KW - Heat-shock

KW - Life span

KW - Longevity

KW - Mutant

UR - http://www.scopus.com/inward/record.url?scp=0033979686&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033979686&partnerID=8YFLogxK

U2 - 10.1016/S0047-6374(99)00100-1

DO - 10.1016/S0047-6374(99)00100-1

M3 - Article

C2 - 10708258

AN - SCOPUS:0033979686

VL - 113

SP - 101

EP - 116

JO - Mechanisms of Ageing and Development

JF - Mechanisms of Ageing and Development

SN - 0047-6374

IS - 2

ER -