Isolated vegetal cortex from Xenopus oocytes selectively retains localized mRNAs

Richard P. Elinson, Mary Lou L King, Caryl Forristall

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The process of converting an egg to a multicellular organism often begins with a polarization of information within the oocyte. The cytoskeleton plays an important role in maintaining such asymmetries. Here we describe the isolation and characterization of cortices, rich in cytoskeletal elements, from the animal and vegetal poles of stage VI Xenopus oocytes. Ultrastructural and immunocytochemical analyses revealed that the 10-μm-thick isolates included a cytokeratin network, mitochondria, and other elements, found in the oocyte cortex. Furthermore, three vegetally localized mRNAs, Vg1, Xcat-2, and Xcat-3, were all found to be specifically retained within the isolated vegetal cortices, in sharp contrast to histone RNA. Our findings support the view that the vegetal cortex represents a unique cytoskeletal domain in which a rare class of maternal mRNAs becomes localized.

Original languageEnglish
Pages (from-to)554-562
Number of pages9
JournalDevelopmental Biology
Volume160
Issue number2
StatePublished - Dec 1 1993

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Xenopus
Oocytes
Messenger RNA
Stored Messenger RNA
Keratins
Cytoskeleton
Histones
Ovum
Mitochondria
RNA

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Elinson, R. P., King, M. L. L., & Forristall, C. (1993). Isolated vegetal cortex from Xenopus oocytes selectively retains localized mRNAs. Developmental Biology, 160(2), 554-562.

Isolated vegetal cortex from Xenopus oocytes selectively retains localized mRNAs. / Elinson, Richard P.; King, Mary Lou L; Forristall, Caryl.

In: Developmental Biology, Vol. 160, No. 2, 01.12.1993, p. 554-562.

Research output: Contribution to journalArticle

Elinson, RP, King, MLL & Forristall, C 1993, 'Isolated vegetal cortex from Xenopus oocytes selectively retains localized mRNAs', Developmental Biology, vol. 160, no. 2, pp. 554-562.
Elinson, Richard P. ; King, Mary Lou L ; Forristall, Caryl. / Isolated vegetal cortex from Xenopus oocytes selectively retains localized mRNAs. In: Developmental Biology. 1993 ; Vol. 160, No. 2. pp. 554-562.
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