TY - JOUR
T1 - Isoform-specific membrane translocation of protein kinase C after ischemic preconditioning
AU - Kurkinen, Kaisa
AU - Busto, Raul
AU - Goldsteins, Gundars
AU - Koistinaho, Jari
AU - Pérez-Pinzón, Miguel A.
PY - 2001/11/14
Y1 - 2001/11/14
N2 - Mild cerebral anoxic/ischemic/stress insults promote 'tolerance' and thereby protect the brain from subsequent 'lethal' anoxic/ischemic insults. We examined whether specific activation of PKC α, δ, ε, or ζ isoforms is associated with ischemic preconditioning (IPC) in rat brain. IPC was produced by a 2-minute global cerebral ischemia. Membrane and cytosolic fractions of the hippocampi were immunoblotted using specific antibodies for PKCα, δ, ε, and ζ. PKCα showed a significant translocation to the membrane fraction from 30 min to 4 h and PKCδ at 4 h following IPC. In contrast, the membrane/cytosol ratio of PKCε showed a tendency to decrease at 30 min and 8 h, and the membrane/cytosol ratio of PKCζ was significantly decreased from 30 min to 24 h following IPC. These findings indicate PKC isoform-specific membrane translocations in the hippocampus after brief global brain ischemia and suggest that activation of PKCα and PKCδ may be associated with IPC-induced tolerance in the rat hippocampus.
AB - Mild cerebral anoxic/ischemic/stress insults promote 'tolerance' and thereby protect the brain from subsequent 'lethal' anoxic/ischemic insults. We examined whether specific activation of PKC α, δ, ε, or ζ isoforms is associated with ischemic preconditioning (IPC) in rat brain. IPC was produced by a 2-minute global cerebral ischemia. Membrane and cytosolic fractions of the hippocampi were immunoblotted using specific antibodies for PKCα, δ, ε, and ζ. PKCα showed a significant translocation to the membrane fraction from 30 min to 4 h and PKCδ at 4 h following IPC. In contrast, the membrane/cytosol ratio of PKCε showed a tendency to decrease at 30 min and 8 h, and the membrane/cytosol ratio of PKCζ was significantly decreased from 30 min to 24 h following IPC. These findings indicate PKC isoform-specific membrane translocations in the hippocampus after brief global brain ischemia and suggest that activation of PKCα and PKCδ may be associated with IPC-induced tolerance in the rat hippocampus.
KW - Adenosine
KW - Anoxia
KW - Ischemia
KW - Tolerance
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U2 - 10.1023/A:1012322906824
DO - 10.1023/A:1012322906824
M3 - Article
C2 - 11700956
AN - SCOPUS:0034764151
VL - 26
SP - 1139
EP - 1144
JO - Neurochemical Research
JF - Neurochemical Research
SN - 0364-3190
IS - 10
ER -