Isoform-specific interactions of apolipoprotein E with the microtubule-associated protein MAP2c: implications for Alzheimer's disease

David Y. Huang, Michel Goedert, Ross Jakes, Karl H. Weisgraber, Craig C. Garner, Ann M. Saunders, Margaret A. Pericak-Vance, Donald E. Schmechel, Allen D. Roses, Warren J. Strittmatter

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

The apolipoprotein E type 4 allele is a susceptibility gene for late-onset Alzheimer's disease. Apolipoprotein E is found in neurons, some of which contain paired helical filaments made of the microtubule-associated protein τ. Previous studies have demonstrated that the apoE3 isoform, but not the apoE4 isoform, binds τ with high avidity. Because the microtubule-associated protein MAP2c also effects microtubule assembly and stability, we examined interactions between apoE isoforms and MAP2c. Similar to the τ-binding results, apoE3, but not apoE4, bound MAP2c. Binding was detectable down to 10-9 M MAP2c and 10-8 M apoE3. Isoform-specific interactions of apoE with the microtubule-associated proteins MAP2c and τ might affect intracellular maintenance of microtubules and could contribute to a time-dependent pathogenesis of Alzheimer's disease.

Original languageEnglish (US)
Pages (from-to)55-58
Number of pages4
JournalNeuroscience Letters
Volume182
Issue number1
DOIs
StatePublished - Nov 21 1994
Externally publishedYes

Keywords

  • Alzheimer's disease
  • Apolipoprotein E
  • MAP2c
  • Microtubule-associated protein

ASJC Scopus subject areas

  • Neuroscience(all)

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