TY - JOUR
T1 - Isoform-specific interactions of apolipoprotein E with the microtubule-associated protein MAP2c
T2 - implications for Alzheimer's disease
AU - Huang, David Y.
AU - Goedert, Michel
AU - Jakes, Ross
AU - Weisgraber, Karl H.
AU - Garner, Craig C.
AU - Saunders, Ann M.
AU - Pericak-Vance, Margaret A.
AU - Schmechel, Donald E.
AU - Roses, Allen D.
AU - Strittmatter, Warren J.
PY - 1994/11/21
Y1 - 1994/11/21
N2 - The apolipoprotein E type 4 allele is a susceptibility gene for late-onset Alzheimer's disease. Apolipoprotein E is found in neurons, some of which contain paired helical filaments made of the microtubule-associated protein τ. Previous studies have demonstrated that the apoE3 isoform, but not the apoE4 isoform, binds τ with high avidity. Because the microtubule-associated protein MAP2c also effects microtubule assembly and stability, we examined interactions between apoE isoforms and MAP2c. Similar to the τ-binding results, apoE3, but not apoE4, bound MAP2c. Binding was detectable down to 10-9 M MAP2c and 10-8 M apoE3. Isoform-specific interactions of apoE with the microtubule-associated proteins MAP2c and τ might affect intracellular maintenance of microtubules and could contribute to a time-dependent pathogenesis of Alzheimer's disease.
AB - The apolipoprotein E type 4 allele is a susceptibility gene for late-onset Alzheimer's disease. Apolipoprotein E is found in neurons, some of which contain paired helical filaments made of the microtubule-associated protein τ. Previous studies have demonstrated that the apoE3 isoform, but not the apoE4 isoform, binds τ with high avidity. Because the microtubule-associated protein MAP2c also effects microtubule assembly and stability, we examined interactions between apoE isoforms and MAP2c. Similar to the τ-binding results, apoE3, but not apoE4, bound MAP2c. Binding was detectable down to 10-9 M MAP2c and 10-8 M apoE3. Isoform-specific interactions of apoE with the microtubule-associated proteins MAP2c and τ might affect intracellular maintenance of microtubules and could contribute to a time-dependent pathogenesis of Alzheimer's disease.
KW - Alzheimer's disease
KW - Apolipoprotein E
KW - MAP2c
KW - Microtubule-associated protein
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U2 - 10.1016/0304-3940(94)90204-6
DO - 10.1016/0304-3940(94)90204-6
M3 - Article
C2 - 7891887
AN - SCOPUS:0028033804
VL - 182
SP - 55
EP - 58
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1
ER -