As a continuation of a study of the molecular events surrounding the establishment of a mature root apex, changes in the pattern and specific activity of the isoenzymes of aminopeptidase (AmP), esterase (EST), glucose‐6‐phosphate de‐hydrogenase (G6PD) and glutamic‐oxaloacetic transaminase (GOT) were investigated during the early germinative growth of the Allium radicle tip. Polyacrylamide disc gel electrophoresis revealed a total of 9 EST fractions. A shift in pattern and reactivity characteristic of pre‐emergence to those characteristic of post‐emergence was demonstrated. G6PD electropherograms displayed 5 isoenzyme bands. Relative specific activity analyses indicated that this enzyme may be more important during the very early stages of germination. Only quantitative differences were noted in the 3 AmP isoenzymes detected. The progressive increase in AmP isoenzyme activity during the period of maximal protein body degradation suggested a role for these exopeptidases in the degradation of reserve proteins. The 3 isoenzyme bands of GOT exhibited only quantitative changes during germination. Parallel increases in the quantity of soluble protein and GOT specific activity suggest that this isoenzyme is involved in the onion's nitrogen metabolism during germination.
|Original language||English (US)|
|Number of pages||7|
|State||Published - Jan 1 1972|
ASJC Scopus subject areas
- Plant Science
- Cell Biology