Isoelectric focusing of bovine major histocompatibility complex class II molecules

David Watkins, J. A. Shadduck, C. E. Rudd, M. E. Stone, H. A. Lewin, N. L. Letvin

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Serological approaches have been relatively unsuccessful in defining the allelic products of the bovine major histocompatibility (MHC) class II loci. We demonstrate that bovine class II allelic products can be characterized by precipitation with a polyclonal antiserum and separation using one-dimensional isoelectric focusing. Polymorphic β chains were present in immunoprecipitates from both biosynthetically and surface-labeled lectin-stimulated bovine T cells. Precipitates from biosynthetically labeled but not surface-labeled T cells contained a basic invariant chain and a non-polymorphic structure. The non-polymorphic structure appears to be a β chain. The polymorphic class II β chain co-segregated with bovine MHC class I allelic products in a half-sibling family, providing evidence for linkage between bovine class I and class II loci. This approach to the biochemical analysis of the bovine class II structures should facilitate the investigation of the association between the bovine products and disease susceptibility.

Original languageEnglish
Pages (from-to)567-570
Number of pages4
JournalEuropean Journal of Immunology
Volume19
Issue number3
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Isoelectric Focusing
Major Histocompatibility Complex
Histocompatibility
Cattle Diseases
T-Lymphocytes
Disease Susceptibility
Lectins
Immune Sera

ASJC Scopus subject areas

  • Immunology

Cite this

Watkins, D., Shadduck, J. A., Rudd, C. E., Stone, M. E., Lewin, H. A., & Letvin, N. L. (1989). Isoelectric focusing of bovine major histocompatibility complex class II molecules. European Journal of Immunology, 19(3), 567-570.

Isoelectric focusing of bovine major histocompatibility complex class II molecules. / Watkins, David; Shadduck, J. A.; Rudd, C. E.; Stone, M. E.; Lewin, H. A.; Letvin, N. L.

In: European Journal of Immunology, Vol. 19, No. 3, 01.01.1989, p. 567-570.

Research output: Contribution to journalArticle

Watkins, D, Shadduck, JA, Rudd, CE, Stone, ME, Lewin, HA & Letvin, NL 1989, 'Isoelectric focusing of bovine major histocompatibility complex class II molecules', European Journal of Immunology, vol. 19, no. 3, pp. 567-570.
Watkins D, Shadduck JA, Rudd CE, Stone ME, Lewin HA, Letvin NL. Isoelectric focusing of bovine major histocompatibility complex class II molecules. European Journal of Immunology. 1989 Jan 1;19(3):567-570.
Watkins, David ; Shadduck, J. A. ; Rudd, C. E. ; Stone, M. E. ; Lewin, H. A. ; Letvin, N. L. / Isoelectric focusing of bovine major histocompatibility complex class II molecules. In: European Journal of Immunology. 1989 ; Vol. 19, No. 3. pp. 567-570.
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