Isoelectric focusing of bovine major histocompatibility complex class II molecules

David I. Watkins, John A. Shadduck, Christopher E. Rudd, Marc E. Stone, Harris A. Lewin, Norman L. Letvin

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Serological approaches have been relatively unsuccessful in defining the allelic products of the bovine major histocompatibility (MHC) class II loci. We demonstrate that bovine class II allelic products can be characterized by precipitation with a polyclonal antiserum and separation using one-dimensional isoelectric focusing. Polymorphic β chains were present in immunoprecipitates from both biosynthetically and surface-labeled lectin-stimulated bovine T cells. Precipitates from biosynthetically labeled but not surface-labeled T cells contained a basic invariant chain and a non-polymorphic structure. The non-polymorphic structure appears to be a β chain. The polymorphic class II β chain co-segregated with bovine MHC class I allelic products in a half-sibling family, providing evidence for linkage between bovine class I and class II loci. This approach to the biochemical analysis of the bovine class II structures should facilitate the investigation of the association between the bovine products and disease susceptibility.

Original languageEnglish (US)
Pages (from-to)567-570
Number of pages4
JournalEuropean Journal of Immunology
Volume19
Issue number3
DOIs
StatePublished - Mar 1989
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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