An isoelectric focusing technique on a Sephadex column is described. This technique provides for the fractionation of amphoteric compounds in a pH gradient and allows for better than 95% recovery of the focused material. Focusing in this system over a pH range of 3.5-10 reaches equilibrium in approximately 5 hr and thereby reduces the exposure of more labile proteins to long focusing times.
ASJC Scopus subject areas
- Molecular Biology