Isoelectric focusing in a Sephadex column

T. J. O'Brien, H. H. Liebke, Herman S Cheung, L. K. Johnson

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Abstract

An isoelectric focusing technique on a Sephadex column is described. This technique provides for the fractionation of amphoteric compounds in a pH gradient and allows for better than 95% recovery of the focused material. Focusing in this system over a pH range of 3.5-10 reaches equilibrium in approximately 5 hr and thereby reduces the exposure of more labile proteins to long focusing times.

Original languageEnglish
Pages (from-to)38-44
Number of pages7
JournalAnalytical Biochemistry
Volume72
Issue number1-2
DOIs
StatePublished - May 7 1976
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Isoelectric focusing in a Sephadex column. / O'Brien, T. J.; Liebke, H. H.; Cheung, Herman S; Johnson, L. K.

In: Analytical Biochemistry, Vol. 72, No. 1-2, 07.05.1976, p. 38-44.

Research output: Contribution to journalArticle