Isoelectric focusing in a Sephadex column

T. J. O'Brien, H. H. Liebke, H. S. Cheung, L. K. Johnson

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

An isoelectric focusing technique on a Sephadex column is described. This technique provides for the fractionation of amphoteric compounds in a pH gradient and allows for better than 95% recovery of the focused material. Focusing in this system over a pH range of 3.5-10 reaches equilibrium in approximately 5 hr and thereby reduces the exposure of more labile proteins to long focusing times.

Original languageEnglish (US)
Pages (from-to)38-44
Number of pages7
JournalAnalytical Biochemistry
Volume72
Issue number1-2
DOIs
StatePublished - May 7 1976
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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