Isoelectric focusing in a Sephadex column

T. J. O'Brien, H. H. Liebke, H. S. Cheung, L. K. Johnson

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


An isoelectric focusing technique on a Sephadex column is described. This technique provides for the fractionation of amphoteric compounds in a pH gradient and allows for better than 95% recovery of the focused material. Focusing in this system over a pH range of 3.5-10 reaches equilibrium in approximately 5 hr and thereby reduces the exposure of more labile proteins to long focusing times.

Original languageEnglish (US)
Pages (from-to)38-44
Number of pages7
JournalAnalytical Biochemistry
Issue number1-2
StatePublished - May 7 1976
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Isoelectric focusing in a Sephadex column'. Together they form a unique fingerprint.

Cite this