Islet cell transplantation: In vivo and in vitro functional assessment of nonhuman primate pancreatic islets

A. Ranuncoli, N. Cautero, Camillo Ricordi, M. Masetti, Ruth Molano, Luca A Inverardi, Rodolfo Alejandro, Norma S Kenyon

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Transplantation of pancreatic islets of Langerhans as a therapeutic approach for treatment of type I diabetes offers an alternative to subcutaneous insulin injections. Normalization of blood glucose levels by transplanted islets may prevent the development of diabetes-related complications. Problems related to rejection, recurrence of autoimmunity, and local inflammation upon transplantation of islets into the liver need to be solved before the implementation of islet cell transplantation can be viewed as a justifiable procedure in a large cohort of patients. Islet cell isolation has been quite successful in small animals, but the translation of this approach to nonhuman primates has been less rewarding. One of the main problems encountered in nonhuman primate models is the difficulty of isolating an adequate number of functional islets for transplantation. The aim of the present study was to develop a method for isolating a sufficient number of viable islets from nonhuman primates to allow for reversal of diabetes. By implementing minor modifications in the automated method for human islet isolation we were able to obtain viable, functional islets that responded normally to glucose stimulation in vitro. These islets were also able to reverse diabetes in immunocompromised nude mice, rendered diabetic by streptozotocin. This method of islet cell isolation has enabled us to proceed with protocols of allogeneic islet cell transplantation in preclinical, nonhuman primate models.

Original languageEnglish
Pages (from-to)409-414
Number of pages6
JournalCell Transplantation
Volume9
Issue number3
StatePublished - Sep 9 2000

Fingerprint

Functional assessment
Islets of Langerhans Transplantation
Cell Transplantation
Medical problems
Islets of Langerhans
Primates
Glucose
Cell Separation
Insulin
Liver
Animals
Blood
Homologous Transplantation
Diabetes Complications
Subcutaneous Injections
Streptozocin
Autoimmunity
Type 1 Diabetes Mellitus
Nude Mice
Blood Glucose

Keywords

  • Assessment
  • In vitro
  • In vivo
  • Nonhuman primate islets
  • Yield

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation

Cite this

Islet cell transplantation : In vivo and in vitro functional assessment of nonhuman primate pancreatic islets. / Ranuncoli, A.; Cautero, N.; Ricordi, Camillo; Masetti, M.; Molano, Ruth; Inverardi, Luca A; Alejandro, Rodolfo; Kenyon, Norma S.

In: Cell Transplantation, Vol. 9, No. 3, 09.09.2000, p. 409-414.

Research output: Contribution to journalArticle

Ranuncoli A, Cautero N, Ricordi C, Masetti M, Molano R, Inverardi LA et al. Islet cell transplantation: In vivo and in vitro functional assessment of nonhuman primate pancreatic islets. Cell Transplantation. 2000 Sep 9;9(3):409-414.
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