Ischemic preconditioning via epsilon protein kinase C activation requires cyclooxygenase-2 activation in vitro

E. Kim, Ami Raval, R. A. DeFazio, Miguel Perez-Pinzon

Research output: Contribution to journalArticle

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Abstract

The signaling pathway of cyclooxygenase-2 (COX-2) induction following ischemic preconditioning (IPC) in brain remains undefined. To determine role of COX-2 in ischemic preconditioning, we used two in vitro models: mixed cortical neuron/astrocyte cell cultures and organotypic hippocampal slice cultures. We simulated IPC by exposing cell or slice cultures to 1 h or 15 min of oxygen/glucose deprivation (OGD), respectively, 48 h prior to ischemia. To mimic ischemia in vitro, we exposed cell or slice cultures to OGD of 4 h or 40 min, respectively. In cell cultures, these experiments revealed that COX-2 induction peaked at 24 h following IPC in cell culture. Inhibition of COX-2 activation with 50 μM NS-398 (a COX-2 selective inhibitor) abolished IPC-mediated neuroprotection in both in vitro models. Next, we tested whether ε protein kinase C (εPKC) and extracellular signal regulated kinase 1/2 (ERK1/2) activation was involved in IPC-mediated neuroprotection and COX-2 expression in cell culture. Cell cultures were treated with an εPKC-specific activating peptide (ψεRACK, 100 nM) for 1 h, and 48 h later were exposed to OGD. εPKC activation increased ERK1/2 phosphorylation and COX-2 induction and conferred neuroprotection similar to IPC. Additionally, inhibition of either εPKC or ERK1/2 activation abolished COX-2 expression and neuroprotection due to ischemic preconditioning. These results demonstrate a crucial role for the εPKC→ERK1/2→COX-2 pathway in the induction of neuroprotection via ischemic preconditioning.

Original languageEnglish
Pages (from-to)931-941
Number of pages11
JournalNeuroscience
Volume145
Issue number3
DOIs
StatePublished - Mar 30 2007

Fingerprint

Protein Kinase C-epsilon
Ischemic Preconditioning
Cyclooxygenase 2
Cell Culture Techniques
Protein Kinase C
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase 1
Oxygen
Glucose
Ischemia
In Vitro Techniques
Cyclooxygenase 2 Inhibitors
Astrocytes
Phosphorylation
Neuroprotection
Neurons

Keywords

  • ERK1/2
  • ischemia
  • mixed cortical neuron/astrocyte cell cultures
  • neuroprotection
  • organotypic hippocampal slice cultures
  • oxygen/glucose deprivation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ischemic preconditioning via epsilon protein kinase C activation requires cyclooxygenase-2 activation in vitro. / Kim, E.; Raval, Ami; DeFazio, R. A.; Perez-Pinzon, Miguel.

In: Neuroscience, Vol. 145, No. 3, 30.03.2007, p. 931-941.

Research output: Contribution to journalArticle

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