Ischemic preconditioning increases antioxidants in the brain and peripheral organs after cerebral ischemia

Lucio Glantz, Aharon Avramovich, Victoria Trembovler, Vladimir Gurvitz, Ron Kohen, Leonid A. Eidelman, Esther Shohami

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Background and purpose. Low molecular weight antioxidants (LMWA), which reflect tissue reducing power, are among the endogenous mechanisms for neutralizing reactive oxygen species (ROS). Ischemic preconditioning (IPC) was associated with decreased oxidative stress. We examined the effect of focal ischemia on LMWA and on prostaglandin E2 (PGE2, a product of arachidonic acid oxidation) in the brain, heart, liver, and lungs of rats subjected to 90 min of ischemia and in IPC rats subjected to similar insult. Methods. Transient right middle cerebral artery occlusion (MCAO) was performed for 90 min and at 0, 5, 30, 60, or 240 min of reperfusion, LMWA and PGE 2 were evaluated by cyclic voltametry (CV) and radioimmunoassay, respectively. IPC was induced by 2 min of MCAO, 24 h prior to the major ischemic episode. Results. LMWA decreased at 5 min of reperfusion in the brain, heart, liver, and lung and rose 4 h later only in the brain. PGE2 levels increased three to fivefold in all tissues examined. Surprisingly, in IPC rats a dramatic increase of LMWA occurred at 5 min of reperfusion in the brain and in the peripheral organs. Uric acid, but not ascorbic, is the major LMWA increased. Conclusions. We propose that after ischemia, ROS rapidly consume the antioxidants reserves in the brain and also in peripheral organs, suggesting that the whole body is under oxidative stress. Moreover, part of the neuroprotection afforded by IPC is mediated by the brain's ability to mobilize antioxidants, especially uric acid, that attenuate the massive ROS-mediated oxidative stress.

Original languageEnglish
Pages (from-to)117-124
Number of pages8
JournalExperimental Neurology
Volume192
Issue number1
DOIs
StatePublished - Mar 1 2005
Externally publishedYes

Fingerprint

Ischemic Preconditioning
Brain Ischemia
Antioxidants
Molecular Weight
Brain
Dinoprostone
Reperfusion
Reactive Oxygen Species
Oxidative Stress
Ischemia
Middle Cerebral Artery Infarction
Uric Acid
Cognitive Reserve
Lung
Aptitude
Liver
Prostaglandins E
Arachidonic Acid
Radioimmunoassay

Keywords

  • Ascorbic acid
  • Biological oxidation potential
  • Brain ischemia
  • Reactive oxygen species
  • Uric acid

ASJC Scopus subject areas

  • Neurology
  • Neuroscience(all)

Cite this

Glantz, L., Avramovich, A., Trembovler, V., Gurvitz, V., Kohen, R., Eidelman, L. A., & Shohami, E. (2005). Ischemic preconditioning increases antioxidants in the brain and peripheral organs after cerebral ischemia. Experimental Neurology, 192(1), 117-124. https://doi.org/10.1016/j.expneurol.2004.11.012

Ischemic preconditioning increases antioxidants in the brain and peripheral organs after cerebral ischemia. / Glantz, Lucio; Avramovich, Aharon; Trembovler, Victoria; Gurvitz, Vladimir; Kohen, Ron; Eidelman, Leonid A.; Shohami, Esther.

In: Experimental Neurology, Vol. 192, No. 1, 01.03.2005, p. 117-124.

Research output: Contribution to journalArticle

Glantz, L, Avramovich, A, Trembovler, V, Gurvitz, V, Kohen, R, Eidelman, LA & Shohami, E 2005, 'Ischemic preconditioning increases antioxidants in the brain and peripheral organs after cerebral ischemia', Experimental Neurology, vol. 192, no. 1, pp. 117-124. https://doi.org/10.1016/j.expneurol.2004.11.012
Glantz, Lucio ; Avramovich, Aharon ; Trembovler, Victoria ; Gurvitz, Vladimir ; Kohen, Ron ; Eidelman, Leonid A. ; Shohami, Esther. / Ischemic preconditioning increases antioxidants in the brain and peripheral organs after cerebral ischemia. In: Experimental Neurology. 2005 ; Vol. 192, No. 1. pp. 117-124.
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