Is urea pulsing in toadfish related to environmental O2 or CO2 levels?

Danielle M Mcdonald, Kathleen M. Gilmour, John F. Barimo, Peter E. Frezza, Patrick J. Walsh, Steve F. Perry

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The neurochemical, serotonin (5-hydroxytryptamine; 5-HT) is involved in the regulation of toadfish pulsatile urea excretion as well as the teleost hypoxia response. Thus, the goal of this study was to determine whether environmental conditions that activate branchial chemoreceptors also trigger pulsatile urea excretion in toadfish, since environmental dissolved oxygen levels in a typical toadfish habitat show significant diel fluctuations, often reaching hypoxic conditions at dawn. Toadfish were fitted with arterial, venous and/or buccal catheters and were exposed to various environmental conditions, and/or injected with the O2 chemoreceptor agonist NaCN or the 5-HT2 receptor agonist α-methyl-5HT. Arterial PO2, as well as ammonia and urea excretion were monitored. Natural fluctuations in arterial PO2 levels in toadfish did not correlate with the occurrence of a urea pulse. Chronic exposure (24 h) of toadfish to hyperoxia was without effect on nitrogen excretion, however, exposure to hypoxia caused a significant reduction in the frequency of urea pulses, and exposure to hypercapnia resulted in a reduction in the percentage of nitrogen waste excreted as urea. Of toadfish exposed acutely to hypoxia, 20% pulsed within 1 h, whereas none pulsed after normoxic or hypercapnic treatments. Furthermore, 20% of fish injected intravenously with NaCN pulsed within 1 h of injection, but no fish pulsed after injection of NaCN into the buccal cavity. To test whether environmental conditions affected 5-HT2 receptors, toadfish were injected with α-methyl-5HT, which elicits urea pulses in toadfish. No significant differences in pulse size occurred among the various environmental treatments. Our findings suggest that neither the environmental conditions of hypoxia, hyperoxia or hypercapnia, nor direct branchial chemoreceptor activation by NaCN play a major role in the regulation of pulsatile urea excretion in toadfish.

Original languageEnglish
Pages (from-to)366-374
Number of pages9
JournalComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Volume146
Issue number3
DOIs
StatePublished - Mar 1 2007

Fingerprint

Batrachoidiformes
Urea
Serotonin
Hyperoxia
Fish
Hypercapnia
Cheek
Nitrogen
Fishes
Serotonin 5-HT2 Receptor Agonists
Catheters
Dissolved oxygen
Ammonia
Injections
Chemical activation
Ecosystem

Keywords

  • Hypercapnia
  • Hyperoxia
  • Hypoxia
  • Nitrogen excretion
  • Opsanus beta
  • tUT
  • Urea transport

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Physiology

Cite this

Is urea pulsing in toadfish related to environmental O2 or CO2 levels? / Mcdonald, Danielle M; Gilmour, Kathleen M.; Barimo, John F.; Frezza, Peter E.; Walsh, Patrick J.; Perry, Steve F.

In: Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, Vol. 146, No. 3, 01.03.2007, p. 366-374.

Research output: Contribution to journalArticle

Mcdonald, Danielle M ; Gilmour, Kathleen M. ; Barimo, John F. ; Frezza, Peter E. ; Walsh, Patrick J. ; Perry, Steve F. / Is urea pulsing in toadfish related to environmental O2 or CO2 levels?. In: Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology. 2007 ; Vol. 146, No. 3. pp. 366-374.
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