Iontophoresis of dexamethasone in the treatment of endotoxin-induced- uveitis in rats

Francine F. Behar-Cohen, Jean-Marie A Parel, Yves Pouliquen, Beatrice Thillaye-Goldenberg, Olivier Goureau, Silke Heydolph, Yves Courtois, Yvonne De Kozak

Research output: Contribution to journalArticle

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Abstract

The purpose of this study was to evaluate the efficacy of a Coulomb Controlled Iontophoresis system (CCI) in the local delivery of corticosteroids for the treatment of uveitis. The therapeutic efficacy of Dexamethasone (Dex) administered by CCI was compared to systemic injection and to topical application with the iontophoresis apparatus in the absence of electrical current. The evaluation was done in the treatment of the endotoxin-induced uveitis (EIU) model, and in the effect on TNF gene expression in the iris/ciliary body as well as in the retina and on TNF levels in aqueous humor and vitreous. Dex was administered either at the time of LPS injection or 5 hours later. For iontophoresis, we used a 1 ml reservoir-electrode covering the cornea, the limbus, and the first millimeter of the sclera. The applied electrical current was of 400 μA during four minutes with a total surface charge of 0.4 C cm-2. EIU was evaluated by clinical examination, by counts of intraocular inflammatory cells on histological sections, and by measuring the protein levels in the aqueous humor and in the vitreous. The TNF-α gene expression in the iris and ciliary body, and in the retina was evaluated by RT-PCR. The systemic effect of Dex delivered by CCI was evaluated on the level of serum TNF-α in EIU. Our results demonstrated that local administration of Dex by CCI inhibited anterior and posterior signs of intraocular inflammation as effectively as systemic administration, with no effect on systemic level of TNF. In the anterior and posterior segments of the eye, the protein exudation, TNF levels and the cellular infiltration were inhibited. The TNF-α gene expression was inhibited in the anterior as well as the posterior segment of the eye. No clinical nor histological damage were caused by the CCI apparatus. In conclusion, CCI administration of Dex allows for a therapeutic effect on the posterior as well as the anterior segment of the eye, and may present a viable alternative to systemic administration of glucocorticoids in severe ocular inflammations.

Original languageEnglish
Pages (from-to)533-545
Number of pages13
JournalExperimental Eye Research
Volume65
Issue number4
DOIs
StatePublished - Oct 1 1997

Fingerprint

Iontophoresis
Uveitis
Endotoxins
Dexamethasone
Ciliary Body
Aqueous Humor
Iris
Therapeutics
Gene Expression
Retina
Eye Proteins
Posterior Eye Segment
Limbus Corneae
Anterior Eye Segment
Inflammation
Sclera
Injections
Therapeutic Uses
Glucocorticoids
Adrenal Cortex Hormones

Keywords

  • Drug-delivery
  • EIU
  • Glucocorticoids
  • Iontophoresis
  • Rat
  • TNF-α

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Behar-Cohen, F. F., Parel, J-M. A., Pouliquen, Y., Thillaye-Goldenberg, B., Goureau, O., Heydolph, S., ... De Kozak, Y. (1997). Iontophoresis of dexamethasone in the treatment of endotoxin-induced- uveitis in rats. Experimental Eye Research, 65(4), 533-545. https://doi.org/10.1006/exer.1997.0364

Iontophoresis of dexamethasone in the treatment of endotoxin-induced- uveitis in rats. / Behar-Cohen, Francine F.; Parel, Jean-Marie A; Pouliquen, Yves; Thillaye-Goldenberg, Beatrice; Goureau, Olivier; Heydolph, Silke; Courtois, Yves; De Kozak, Yvonne.

In: Experimental Eye Research, Vol. 65, No. 4, 01.10.1997, p. 533-545.

Research output: Contribution to journalArticle

Behar-Cohen, FF, Parel, J-MA, Pouliquen, Y, Thillaye-Goldenberg, B, Goureau, O, Heydolph, S, Courtois, Y & De Kozak, Y 1997, 'Iontophoresis of dexamethasone in the treatment of endotoxin-induced- uveitis in rats', Experimental Eye Research, vol. 65, no. 4, pp. 533-545. https://doi.org/10.1006/exer.1997.0364
Behar-Cohen FF, Parel J-MA, Pouliquen Y, Thillaye-Goldenberg B, Goureau O, Heydolph S et al. Iontophoresis of dexamethasone in the treatment of endotoxin-induced- uveitis in rats. Experimental Eye Research. 1997 Oct 1;65(4):533-545. https://doi.org/10.1006/exer.1997.0364
Behar-Cohen, Francine F. ; Parel, Jean-Marie A ; Pouliquen, Yves ; Thillaye-Goldenberg, Beatrice ; Goureau, Olivier ; Heydolph, Silke ; Courtois, Yves ; De Kozak, Yvonne. / Iontophoresis of dexamethasone in the treatment of endotoxin-induced- uveitis in rats. In: Experimental Eye Research. 1997 ; Vol. 65, No. 4. pp. 533-545.
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