Ionoregulatory strategies and the role of urea in the Magadi tilapia (Alcolapia grahami)

Chris M. Wood, Paul Wilson, Harold L. Bergman, Annie N. Bergman, Pierre Laurent, George Otiang'a-Owiti, Patrick J. Walsh

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The unique ureotelic tilapia Alcolapia grahami lives in the highly alkaline and saline waters of Lake Magadi, Kenya (pH ∼10.0, alkalinity ∼380 mmol·L-1, Na+ ∼350 mmol·L-1, Cl- ∼110 mmol·L-1, osmolality ∼580 mosmol·kg-1). In 100% lake water, the Magadi tilapia maintained plasma Na+, Cl-, and osmolality at levels typical of marine teleosts and drank the medium at 8.01 ± 1.29 mL·kg-1·h-1. Gill chloride cells were predominantly of the sea-water type (recessed, with apical pits) but a few freshwater-type chloride cells (surficial, with flat apical exposure) were also present. Whole-body Na+ and Cl- concentrations were relatively high and exhibited larger relative changes in response to salinity transfers than did plasma ions. All fish succumbed upon acute transfer to 1% lake water, but tolerated acute transfer to 10% lake water well, and gradual long-term acclimation to both 10 and 1% lake water without change in plasma cortisol. Plasma osmolytes were here maintained at levels typical of freshwater teleosts. Curiously, drinking continued at the same rate in fish adapted to 1% lake water, but chloride cells were now exclusively of the freshwater type. Significant mortality and elevated cortisol occurred after acute transfer to 200% lake water. However, the fish survived well during gradual adaptation to 200% lake water, although plasma cortisol remained chronically elevated. Urea levels accounted for only 2-3% of internal osmolality in 100% lake water but responded to a greater extent than plasma ions during exposure to 10 and 200% lake water, decreasing by 28-42% in the former and increasing by over 500% in the latter relative to simultaneous-control values. Urea thereby played a small but significant role (up to 8% of internal osmolality) in osmoregulation.

Original languageEnglish (US)
Pages (from-to)503-515
Number of pages13
JournalCanadian journal of zoology
Volume80
Issue number3
DOIs
StatePublished - May 27 2002

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Animal Science and Zoology

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    Wood, C. M., Wilson, P., Bergman, H. L., Bergman, A. N., Laurent, P., Otiang'a-Owiti, G., & Walsh, P. J. (2002). Ionoregulatory strategies and the role of urea in the Magadi tilapia (Alcolapia grahami). Canadian journal of zoology, 80(3), 503-515. https://doi.org/10.1139/z02-019