Abstract
L-Glutamate (L-GLU) induced astrocyte swelling in a time- and concentration-dependent, as well as Na+and Ca2+-dependent, and Cl- independent manner. Swelling was prevented by MK-801, cystine, and ouabain. Since L-GLU swelling is ionically dependent, we determined the role of various ions in such swelling. Our results indicate that K+ uptake plays a major role in the mechanism of L-GLU-induced astrocyte swelling. Like swelling, K+ uptake is dependent on Ca2+ and Na+, but not on Cl-. Likewise, K+ uptake was inhibited by MK-801, cystine, and ouabain. The K+ channel blockers, Ba2+ and tetraethylammonium, partially prevented L-GLU- induced swelling. In addition to K+ channels, K+ influx may also be mediated through Na+/K+-ATPase, as its activity is increased by L-GLU uptake along with the required Na+. Taken together, the data suggest that K+ influx plays a key role in the mechanism of L-GLU-mediated astrocyte swelling.
| Original language | English |
|---|---|
| Pages (from-to) | 307-321 |
| Number of pages | 15 |
| Journal | Journal of Neuroscience Research |
| Volume | 52 |
| Issue number | 3 |
| DOIs | |
| State | Published - May 1 1998 |
Fingerprint
Keywords
- Astrocyte
- Calcium
- Cystine
- Glutamate swelling
- MK-801
- Potassium uptake
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Ionic mechanisms in glutamate-induced astrocyte swelling : Role of K+ influx. / Bender, Alex S.; Schousboe, Arne; Reichelt, Winfried; Norenberg, Michael D.
In: Journal of Neuroscience Research, Vol. 52, No. 3, 01.05.1998, p. 307-321.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ionic mechanisms in glutamate-induced astrocyte swelling
T2 - Role of K+ influx
AU - Bender, Alex S.
AU - Schousboe, Arne
AU - Reichelt, Winfried
AU - Norenberg, Michael D
PY - 1998/5/1
Y1 - 1998/5/1
N2 - L-Glutamate (L-GLU) induced astrocyte swelling in a time- and concentration-dependent, as well as Na+and Ca2+-dependent, and Cl- independent manner. Swelling was prevented by MK-801, cystine, and ouabain. Since L-GLU swelling is ionically dependent, we determined the role of various ions in such swelling. Our results indicate that K+ uptake plays a major role in the mechanism of L-GLU-induced astrocyte swelling. Like swelling, K+ uptake is dependent on Ca2+ and Na+, but not on Cl-. Likewise, K+ uptake was inhibited by MK-801, cystine, and ouabain. The K+ channel blockers, Ba2+ and tetraethylammonium, partially prevented L-GLU- induced swelling. In addition to K+ channels, K+ influx may also be mediated through Na+/K+-ATPase, as its activity is increased by L-GLU uptake along with the required Na+. Taken together, the data suggest that K+ influx plays a key role in the mechanism of L-GLU-mediated astrocyte swelling.
AB - L-Glutamate (L-GLU) induced astrocyte swelling in a time- and concentration-dependent, as well as Na+and Ca2+-dependent, and Cl- independent manner. Swelling was prevented by MK-801, cystine, and ouabain. Since L-GLU swelling is ionically dependent, we determined the role of various ions in such swelling. Our results indicate that K+ uptake plays a major role in the mechanism of L-GLU-induced astrocyte swelling. Like swelling, K+ uptake is dependent on Ca2+ and Na+, but not on Cl-. Likewise, K+ uptake was inhibited by MK-801, cystine, and ouabain. The K+ channel blockers, Ba2+ and tetraethylammonium, partially prevented L-GLU- induced swelling. In addition to K+ channels, K+ influx may also be mediated through Na+/K+-ATPase, as its activity is increased by L-GLU uptake along with the required Na+. Taken together, the data suggest that K+ influx plays a key role in the mechanism of L-GLU-mediated astrocyte swelling.
KW - Astrocyte
KW - Calcium
KW - Cystine
KW - Glutamate swelling
KW - MK-801
KW - Potassium uptake
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UR - http://www.scopus.com/inward/citedby.url?scp=0032080435&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-4547(19980501)52:3<307::AID-JNR7>3.0.CO;2-H
DO - 10.1002/(SICI)1097-4547(19980501)52:3<307::AID-JNR7>3.0.CO;2-H
M3 - Article
C2 - 9590439
AN - SCOPUS:0032080435
VL - 52
SP - 307
EP - 321
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
SN - 0360-4012
IS - 3
ER -