Ionic channels in the plasma membrane of Schizosaccharomyces pombe: Evidence from patch-clamp measurements

Vladimír Vacata; Milan Höfer; H. Peter Larsson; Harold Lecar

doi:10.1007/BF00768067

Ionic channels in the plasma membrane of Schizosaccharomyces pombe: Evidence from patch-clamp measurements

Vladimír Vacata, Milan Höfer, H. Peter Larsson, Harold Lecar

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Patch-clamp studies of the yeast Schizosaccharomyces pombe reveal that the plasma membrane contains a voltage-gated channel mildly selective for potassium over sodium, lithium, and chloride. The channel exhibits several conductances with a maximum of 153 pS. The channel gates in the region of physiologically relevant voltages, being closed at hyperpolarizing and open at depolarizing voltages. It is not inhibited by tetraethylammonium, quinine, or quinidine applied from the cytoplasmic side of the membrane; similarly, ATP and stretch have no effect. The frequency of its occurrence in patches implies that about 35 channels of this kind are present in the plasma membrane of a single cell.

Original language	English (US)
Pages (from-to)	43-53
Number of pages	11
Journal	Journal of Bioenergetics and Biomembranes
Volume	25
Issue number	1
DOIs	https://doi.org/10.1007/BF00768067
State	Published - Feb 1 1993
Externally published	Yes

Keywords

channel gating
density of channels
ionic selectivity
multilevel conductance
Plasma membrane channels
Schizosaccharomyces pombe

ASJC Scopus subject areas

Physiology
Cell Biology

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10.1007/BF00768067

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abstract = "Patch-clamp studies of the yeast Schizosaccharomyces pombe reveal that the plasma membrane contains a voltage-gated channel mildly selective for potassium over sodium, lithium, and chloride. The channel exhibits several conductances with a maximum of 153 pS. The channel gates in the region of physiologically relevant voltages, being closed at hyperpolarizing and open at depolarizing voltages. It is not inhibited by tetraethylammonium, quinine, or quinidine applied from the cytoplasmic side of the membrane; similarly, ATP and stretch have no effect. The frequency of its occurrence in patches implies that about 35 channels of this kind are present in the plasma membrane of a single cell.",

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AU - Vacata, Vladimír

AU - Höfer, Milan

AU - Larsson, H. Peter

AU - Lecar, Harold

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