Abstract
Patch-clamp studies of the yeast Schizosaccharomyces pombe reveal that the plasma membrane contains a voltage-gated channel mildly selective for potassium over sodium, lithium, and chloride. The channel exhibits several conductances with a maximum of 153 pS. The channel gates in the region of physiologically relevant voltages, being closed at hyperpolarizing and open at depolarizing voltages. It is not inhibited by tetraethylammonium, quinine, or quinidine applied from the cytoplasmic side of the membrane; similarly, ATP and stretch have no effect. The frequency of its occurrence in patches implies that about 35 channels of this kind are present in the plasma membrane of a single cell.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 43-53 |
| Number of pages | 11 |
| Journal | Journal of Bioenergetics and Biomembranes |
| Volume | 25 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 1 1993 |
| Externally published | Yes |
Keywords
- channel gating
- density of channels
- ionic selectivity
- multilevel conductance
- Plasma membrane channels
- Schizosaccharomyces pombe
ASJC Scopus subject areas
- Physiology
- Cell Biology