TY - JOUR
T1 - Ion-selective microelectrodes suitable for recording rapid changes in extracellular ion concentration
AU - Wen, Rong
AU - Oakley, Burks
N1 - Funding Information:
We thank Dr. Bret Hughes for showing us how to clamp theta tubing during the silanization process. We thank Yiwen Li and Jeffrey Tucker for expert technical assistance. This work was supported by grant EY04364 from the National Eye Institute, U.S. Public Health Service.
PY - 1990/3
Y1 - 1990/3
N2 - A method for fabricating double-barrel, ion-selective microelectrodes with fine tips (0.5-1.5 μm) and rapid response times is described. When made into K+-selective microelectrodes, the electrodes respond to changes in [K+]o with a time constant of 70-95 ms. The electrical response of these electrodes to common-mode voltages can be made to have a time constant of < 2 ms, which minimizes electrical artifacts from field potentials. The application of these microelectrodes to the measurement of rapid, transient changes in retinal [K+]o is presented.
AB - A method for fabricating double-barrel, ion-selective microelectrodes with fine tips (0.5-1.5 μm) and rapid response times is described. When made into K+-selective microelectrodes, the electrodes respond to changes in [K+]o with a time constant of 70-95 ms. The electrical response of these electrodes to common-mode voltages can be made to have a time constant of < 2 ms, which minimizes electrical artifacts from field potentials. The application of these microelectrodes to the measurement of rapid, transient changes in retinal [K+]o is presented.
KW - Electroretinogram b-wave
KW - Extracellular potassium
KW - K-selective microelectrode
KW - Vertebrate retina
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U2 - 10.1016/0165-0270(90)90112-S
DO - 10.1016/0165-0270(90)90112-S
M3 - Article
C2 - 2329840
AN - SCOPUS:0025008903
VL - 31
SP - 207
EP - 213
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
SN - 0165-0270
IS - 3
ER -