Iodine deficiency activates antioxidant genes and causes DNA damage in the thyroid gland of rats and mice

J. Maier, H. van Steeg, C. van Oostrom, R. Paschke, Roy E Weiss, K. Krohn

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Because thyroid nodules are frequent in areas with iodine deficiency the aim of this study was to characterise molecular events during iodine deficiency that could explain mutagenesis and nodule formation. We therefore studied gene expression of catalytic enzymes prominent for H2O2 detoxification and antioxidative defence, quantified DNA oxidation and damage as well as spontaneous mutation rates (SMR) in mice and rats fed an iodine controlled diet. Antioxidative enzymes such as superoxide dismutase 3, glutathione peroxidase 4 and the peroxiredoxins 3 and 5 showed increased mRNA expression, which indicates increased radical burden that could be the cause of additional oxidized base adducts found in thyroidal genomic DNA in our experiments of iodine deficiency. Furthermore, the uracil content of thyroid DNA was significantly higher in the iodine-deficient compared to the control group. While SMR is very high in the normal thyroid gland it is not changed in experimental iodine deficiency. Our data suggest that iodine restriction causes oxidative stress and DNA modifications. A higher uracil content of the thyroid DNA could be a precondition for C → T transitions often detected as somatic mutations in nodular thyroid tissue. However, the absence of increased SMR would argue for more efficient DNA repair in response to iodine restriction.

Original languageEnglish (US)
Pages (from-to)990-999
Number of pages10
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1773
Issue number6
DOIs
StatePublished - Jun 2007
Externally publishedYes

Fingerprint

Iodine
DNA Damage
Thyroid Gland
Antioxidants
Genes
Mutation Rate
Uracil
DNA
phospholipid-hydroperoxide glutathione peroxidase
Peroxiredoxins
Thyroid Nodule
Enzymes
Mutagenesis
DNA Repair
Superoxide Dismutase
Oxidative Stress
Diet
Gene Expression
Control Groups
Messenger RNA

Keywords

  • Comet Assay
  • DNA Damage
  • Iodine Deficiency
  • Mutagenesis

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Iodine deficiency activates antioxidant genes and causes DNA damage in the thyroid gland of rats and mice. / Maier, J.; van Steeg, H.; van Oostrom, C.; Paschke, R.; Weiss, Roy E; Krohn, K.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1773, No. 6, 06.2007, p. 990-999.

Research output: Contribution to journalArticle

Maier, J. ; van Steeg, H. ; van Oostrom, C. ; Paschke, R. ; Weiss, Roy E ; Krohn, K. / Iodine deficiency activates antioxidant genes and causes DNA damage in the thyroid gland of rats and mice. In: Biochimica et Biophysica Acta - Molecular Cell Research. 2007 ; Vol. 1773, No. 6. pp. 990-999.
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