Involvement of Tcf/Lef in establishing cell types along the animal-vegetal axis of sea urchins

Ling Huang; Xiaotao Li; Heithem M. El-Hodiri; Sandeep Dayal; Athula H. Wikramanayake; William H. Klein

doi:10.1007/s004270050013

Involvement of Tcf/Lef in establishing cell types along the animal-vegetal axis of sea urchins

Ling Huang, Xiaotao Li, Heithem M. El-Hodiri, Sandeep Dayal, Athula H. Wikramanayake, William H. Klein

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

Members of the Tcf/Lef family interact with β-catenin to activate programs of gene expression during development. Recently β-catenin was shown to be essential for establishing cell fate along the animal-vegetal axis of the sea urchin embryo. To examine the role of Tcf/Lef in sea urchins we cloned a Strongylocentrotus purpuratus Tcf/Lef homolog. Expression of SpTcf/Lef was maximal when β-catenin became localized to nuclei of vegetal blastomeres, consistent with its acting in combination with β-catenin to specify vegetal cell fates. Expression of a dominant-negative SpTcf/Lef inhibited primary and secondary mesenchyma, endoderm, and aboral ectoderm formation in a manner similar to that observed when nuclear accumulation of β-catenin was prevented. Our results suggest that SpTcf/Lef functions by interacting with β-catenin to specify cell fates along the sea urchin animal-vegetal axis.

Original language	English (US)
Pages (from-to)	73-81
Number of pages	9
Journal	Development Genes and Evolution
Volume	210
Issue number	2
DOIs	https://doi.org/10.1007/s004270050013
State	Published - 2000
Externally published	Yes

Keywords

β-Catenin
Cell fate determination
Sea urchins embryos
Tcf/Lef

ASJC Scopus subject areas

Developmental Biology
Cell Biology
Genetics
Ecology, Evolution, Behavior and Systematics

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title = "Involvement of Tcf/Lef in establishing cell types along the animal-vegetal axis of sea urchins",

abstract = "Members of the Tcf/Lef family interact with β-catenin to activate programs of gene expression during development. Recently β-catenin was shown to be essential for establishing cell fate along the animal-vegetal axis of the sea urchin embryo. To examine the role of Tcf/Lef in sea urchins we cloned a Strongylocentrotus purpuratus Tcf/Lef homolog. Expression of SpTcf/Lef was maximal when β-catenin became localized to nuclei of vegetal blastomeres, consistent with its acting in combination with β-catenin to specify vegetal cell fates. Expression of a dominant-negative SpTcf/Lef inhibited primary and secondary mesenchyma, endoderm, and aboral ectoderm formation in a manner similar to that observed when nuclear accumulation of β-catenin was prevented. Our results suggest that SpTcf/Lef functions by interacting with β-catenin to specify cell fates along the sea urchin animal-vegetal axis.",

keywords = "β-Catenin, Cell fate determination, Sea urchins embryos, Tcf/Lef",

author = "Ling Huang and Xiaotao Li and El-Hodiri, {Heithem M.} and Sandeep Dayal and Wikramanayake, {Athula H.} and Klein, {William H.}",

note = "Funding Information: Acknowledgements We thank Sheng Zhao for assistance in the tree analysis. This work was supported by an NIH grant (HD22619) and the Welch Foundation. We acknowledge the use of M.D. Anderson{\textquoteright}s DNA sequencing core facility, which is supported by NCI Support Grant CA16672.",

year = "2000",

doi = "10.1007/s004270050013",

language = "English (US)",

volume = "210",

pages = "73--81",

journal = "Development Genes and Evolution",

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publisher = "Springer Verlag",

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T1 - Involvement of Tcf/Lef in establishing cell types along the animal-vegetal axis of sea urchins

AU - Huang, Ling

AU - Li, Xiaotao

AU - El-Hodiri, Heithem M.

AU - Dayal, Sandeep

AU - Wikramanayake, Athula H.

AU - Klein, William H.

N1 - Funding Information: Acknowledgements We thank Sheng Zhao for assistance in the tree analysis. This work was supported by an NIH grant (HD22619) and the Welch Foundation. We acknowledge the use of M.D. Anderson’s DNA sequencing core facility, which is supported by NCI Support Grant CA16672.

PY - 2000

Y1 - 2000

N2 - Members of the Tcf/Lef family interact with β-catenin to activate programs of gene expression during development. Recently β-catenin was shown to be essential for establishing cell fate along the animal-vegetal axis of the sea urchin embryo. To examine the role of Tcf/Lef in sea urchins we cloned a Strongylocentrotus purpuratus Tcf/Lef homolog. Expression of SpTcf/Lef was maximal when β-catenin became localized to nuclei of vegetal blastomeres, consistent with its acting in combination with β-catenin to specify vegetal cell fates. Expression of a dominant-negative SpTcf/Lef inhibited primary and secondary mesenchyma, endoderm, and aboral ectoderm formation in a manner similar to that observed when nuclear accumulation of β-catenin was prevented. Our results suggest that SpTcf/Lef functions by interacting with β-catenin to specify cell fates along the sea urchin animal-vegetal axis.

AB - Members of the Tcf/Lef family interact with β-catenin to activate programs of gene expression during development. Recently β-catenin was shown to be essential for establishing cell fate along the animal-vegetal axis of the sea urchin embryo. To examine the role of Tcf/Lef in sea urchins we cloned a Strongylocentrotus purpuratus Tcf/Lef homolog. Expression of SpTcf/Lef was maximal when β-catenin became localized to nuclei of vegetal blastomeres, consistent with its acting in combination with β-catenin to specify vegetal cell fates. Expression of a dominant-negative SpTcf/Lef inhibited primary and secondary mesenchyma, endoderm, and aboral ectoderm formation in a manner similar to that observed when nuclear accumulation of β-catenin was prevented. Our results suggest that SpTcf/Lef functions by interacting with β-catenin to specify cell fates along the sea urchin animal-vegetal axis.

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Involvement of Tcf/Lef in establishing cell types along the animal-vegetal axis of sea urchins

Abstract

Keywords

ASJC Scopus subject areas

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