Abstract
Within hours of Listeria monocytogenes infection, host cell actin filaments form a dense cloud around the intracytoplasmic bacteria and then rearrange to form a polarized comet tail that is associated with moving bacteria. We have devised a cell-free extract system capable of faithfully reconstituting L. monocytogenes motility, and we have used this system to demonstrate that profilin, a host actin monomer-binding protein, is necessary for bacterial actin-based motility. We find that extracts from which profilin has been depleted do not support comet tail formation or bacterial motility. In extracts and host cells, profilin is localized to the back half of the surface of motile L. monocytogenes, the site of actin filament assembly in the tail. This association is not observed with L. monocytogenes mutants that do not express the ActA protein, a bacterial gene product necessary for motility and virulence. Profilin also fails to bind L. monocytogenes grown outside of host cytoplasm, suggesting that at least one other host cell factor is required for this association.
| Original language | English |
|---|---|
| Pages (from-to) | 505-517 |
| Number of pages | 13 |
| Journal | Cell |
| Volume | 76 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 2 1994 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
Cite this
Involvement of profilin in the actin-based motility of L. monocytogenes in cells and in cell-free extracts. / Theriot, Julie A.; Rosenblatt, Jody; Portnoy, Daniel A.; Goldschmidt-Clermont, Pascal; Mitchisont, Timothy J.
In: Cell, Vol. 76, No. 3, 02.03.1994, p. 505-517.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Involvement of profilin in the actin-based motility of L. monocytogenes in cells and in cell-free extracts
AU - Theriot, Julie A.
AU - Rosenblatt, Jody
AU - Portnoy, Daniel A.
AU - Goldschmidt-Clermont, Pascal
AU - Mitchisont, Timothy J.
PY - 1994/3/2
Y1 - 1994/3/2
N2 - Within hours of Listeria monocytogenes infection, host cell actin filaments form a dense cloud around the intracytoplasmic bacteria and then rearrange to form a polarized comet tail that is associated with moving bacteria. We have devised a cell-free extract system capable of faithfully reconstituting L. monocytogenes motility, and we have used this system to demonstrate that profilin, a host actin monomer-binding protein, is necessary for bacterial actin-based motility. We find that extracts from which profilin has been depleted do not support comet tail formation or bacterial motility. In extracts and host cells, profilin is localized to the back half of the surface of motile L. monocytogenes, the site of actin filament assembly in the tail. This association is not observed with L. monocytogenes mutants that do not express the ActA protein, a bacterial gene product necessary for motility and virulence. Profilin also fails to bind L. monocytogenes grown outside of host cytoplasm, suggesting that at least one other host cell factor is required for this association.
AB - Within hours of Listeria monocytogenes infection, host cell actin filaments form a dense cloud around the intracytoplasmic bacteria and then rearrange to form a polarized comet tail that is associated with moving bacteria. We have devised a cell-free extract system capable of faithfully reconstituting L. monocytogenes motility, and we have used this system to demonstrate that profilin, a host actin monomer-binding protein, is necessary for bacterial actin-based motility. We find that extracts from which profilin has been depleted do not support comet tail formation or bacterial motility. In extracts and host cells, profilin is localized to the back half of the surface of motile L. monocytogenes, the site of actin filament assembly in the tail. This association is not observed with L. monocytogenes mutants that do not express the ActA protein, a bacterial gene product necessary for motility and virulence. Profilin also fails to bind L. monocytogenes grown outside of host cytoplasm, suggesting that at least one other host cell factor is required for this association.
UR - http://www.scopus.com/inward/record.url?scp=0028173688&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028173688&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(94)90114-7
DO - 10.1016/0092-8674(94)90114-7
M3 - Article
C2 - 8313471
AN - SCOPUS:0028173688
VL - 76
SP - 505
EP - 517
JO - Cell
JF - Cell
SN - 0092-8674
IS - 3
ER -