Involvement of profilin in the actin-based motility of L. monocytogenes in cells and in cell-free extracts

Julie A. Theriot, Jody Rosenblatt, Daniel A. Portnoy, Pascal Goldschmidt-Clermont, Timothy J. Mitchisont

Research output: Contribution to journalArticle

231 Citations (Scopus)

Abstract

Within hours of Listeria monocytogenes infection, host cell actin filaments form a dense cloud around the intracytoplasmic bacteria and then rearrange to form a polarized comet tail that is associated with moving bacteria. We have devised a cell-free extract system capable of faithfully reconstituting L. monocytogenes motility, and we have used this system to demonstrate that profilin, a host actin monomer-binding protein, is necessary for bacterial actin-based motility. We find that extracts from which profilin has been depleted do not support comet tail formation or bacterial motility. In extracts and host cells, profilin is localized to the back half of the surface of motile L. monocytogenes, the site of actin filament assembly in the tail. This association is not observed with L. monocytogenes mutants that do not express the ActA protein, a bacterial gene product necessary for motility and virulence. Profilin also fails to bind L. monocytogenes grown outside of host cytoplasm, suggesting that at least one other host cell factor is required for this association.

Original languageEnglish
Pages (from-to)505-517
Number of pages13
JournalCell
Volume76
Issue number3
DOIs
StatePublished - Mar 2 1994
Externally publishedYes

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Profilins
Listeria monocytogenes
Cell Extracts
Actins
Bacteria
Actin Cytoskeleton
Listeria
Bacterial Proteins
Microfilament Proteins
Listeriosis
Cell-Free System
Carrier Proteins
Monomers
Virulence
Cytoplasm
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Theriot, J. A., Rosenblatt, J., Portnoy, D. A., Goldschmidt-Clermont, P., & Mitchisont, T. J. (1994). Involvement of profilin in the actin-based motility of L. monocytogenes in cells and in cell-free extracts. Cell, 76(3), 505-517. https://doi.org/10.1016/0092-8674(94)90114-7

Involvement of profilin in the actin-based motility of L. monocytogenes in cells and in cell-free extracts. / Theriot, Julie A.; Rosenblatt, Jody; Portnoy, Daniel A.; Goldschmidt-Clermont, Pascal; Mitchisont, Timothy J.

In: Cell, Vol. 76, No. 3, 02.03.1994, p. 505-517.

Research output: Contribution to journalArticle

Theriot, JA, Rosenblatt, J, Portnoy, DA, Goldschmidt-Clermont, P & Mitchisont, TJ 1994, 'Involvement of profilin in the actin-based motility of L. monocytogenes in cells and in cell-free extracts', Cell, vol. 76, no. 3, pp. 505-517. https://doi.org/10.1016/0092-8674(94)90114-7
Theriot JA, Rosenblatt J, Portnoy DA, Goldschmidt-Clermont P, Mitchisont TJ. Involvement of profilin in the actin-based motility of L. monocytogenes in cells and in cell-free extracts. Cell. 1994 Mar 2;76(3):505-517. https://doi.org/10.1016/0092-8674(94)90114-7
Theriot, Julie A. ; Rosenblatt, Jody ; Portnoy, Daniel A. ; Goldschmidt-Clermont, Pascal ; Mitchisont, Timothy J. / Involvement of profilin in the actin-based motility of L. monocytogenes in cells and in cell-free extracts. In: Cell. 1994 ; Vol. 76, No. 3. pp. 505-517.
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