Involvement of profilin in the actin-based motility of L. monocytogenes in cells and in cell-free extracts

Julie A. Theriot, Jody Rosenblatt, Daniel A. Portnoy, Pascal J. Goldschmidt-Clermont, Timothy J. Mitchison

Research output: Contribution to journalArticlepeer-review

244 Scopus citations


Within hours of Listeria monocytogenes infection, host cell actin filaments form a dense cloud around the intracytoplasmic bacteria and then rearrange to form a polarized comet tail that is associated with moving bacteria. We have devised a cell-free extract system capable of faithfully reconstituting L. monocytogenes motility, and we have used this system to demonstrate that profilin, a host actin monomer-binding protein, is necessary for bacterial actin-based motility. We find that extracts from which profilin has been depleted do not support comet tail formation or bacterial motility. In extracts and host cells, profilin is localized to the back half of the surface of motile L. monocytogenes, the site of actin filament assembly in the tail. This association is not observed with L. monocytogenes mutants that do not express the ActA protein, a bacterial gene product necessary for motility and virulence. Profilin also fails to bind L. monocytogenes grown outside of host cytoplasm, suggesting that at least one other host cell factor is required for this association.

Original languageEnglish (US)
Pages (from-to)505-517
Number of pages13
Issue number3
StatePublished - Feb 11 1994

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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