Involvement of IL-9 in the bronchial phenotype of patients with nasal polyposis

Anne Tsicopoulos, Ayako Shimbara, Patricia De Nadai, Oday Aldewachi, Catherine Lamblin, Philippe Lassalle, Andrew F. Walls, Stéphanie Sénéchal, Roy C Levitt, Jean Darras, Qutayba Hamid, Benoît Wallaert

Research output: Contribution to journalArticle

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Abstract

Background: Nasal polyposis (NP) is frequently associated with asthma. In this disease, asymptomatic bronchial hyperresponsiveness (BHR) is thought to precede the development of asthma. IL-9 and its receptor have been reported as candidate genes for asthma and to be associated with BHR. Objective: The objective of this study was to assess the contribution of IL-9 to the pathogenesis of BHR in NP by comparing the expression of IL-9 and its receptor in bronchial biopsy specimens from three groups of patients with NP:NP without BHR, NP with asymptomatic BHR, and NP with BHR and asthma. Methods: Bronchial biopsy specimens were examined in terms of cellular infiltration and in terms of expression of IL-9 protein and mRNA as well as of its receptor by using immunohistochemistry and in situ hybridization. Results: Patients with NP with asthma as compared with the two other groups exhibited an increased bronchial infiltration of basophils, eosinophils, and T cells that correlated with the asthma score. The two groups of patients with NP with BHR showed an increased expression in IL-9 protein and mRNA as well as an increase in the expression of IL-9R mRNA at the epithelial level. These modifications were inversely correlated with the airway responsiveness to methacholine, producing a 20% fall in FEV1. There was a close association between IL-9+ cells, IL-5 mRNA expression, and eosinophil infiltration that correlated with each other. Conclusions: These results suggest an important role for IL-9 in the pathogenesis of BHR and a causal relation between IL-9 and the development of bronchial eosinophilia in asthma.

Original languageEnglish
Pages (from-to)462-469
Number of pages8
JournalJournal of Allergy and Clinical Immunology
Volume113
Issue number3
DOIs
StatePublished - Mar 1 2004
Externally publishedYes

Fingerprint

Interleukin-9
Nose
Asthma
Phenotype
Interleukin-9 Receptors
Messenger RNA
Eosinophils
Biopsy
Basophils
Methacholine Chloride
Interleukin-5
Eosinophilia
In Situ Hybridization
Proteins
Immunohistochemistry
T-Lymphocytes

Keywords

  • Asthma
  • Bronchial biopsy
  • Bronchial hyperresponsiveness
  • Interleukin-9
  • Nasal polyposis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Tsicopoulos, A., Shimbara, A., De Nadai, P., Aldewachi, O., Lamblin, C., Lassalle, P., ... Wallaert, B. (2004). Involvement of IL-9 in the bronchial phenotype of patients with nasal polyposis. Journal of Allergy and Clinical Immunology, 113(3), 462-469. https://doi.org/10.1016/j.jaci.2003.12.009

Involvement of IL-9 in the bronchial phenotype of patients with nasal polyposis. / Tsicopoulos, Anne; Shimbara, Ayako; De Nadai, Patricia; Aldewachi, Oday; Lamblin, Catherine; Lassalle, Philippe; Walls, Andrew F.; Sénéchal, Stéphanie; Levitt, Roy C; Darras, Jean; Hamid, Qutayba; Wallaert, Benoît.

In: Journal of Allergy and Clinical Immunology, Vol. 113, No. 3, 01.03.2004, p. 462-469.

Research output: Contribution to journalArticle

Tsicopoulos, A, Shimbara, A, De Nadai, P, Aldewachi, O, Lamblin, C, Lassalle, P, Walls, AF, Sénéchal, S, Levitt, RC, Darras, J, Hamid, Q & Wallaert, B 2004, 'Involvement of IL-9 in the bronchial phenotype of patients with nasal polyposis', Journal of Allergy and Clinical Immunology, vol. 113, no. 3, pp. 462-469. https://doi.org/10.1016/j.jaci.2003.12.009
Tsicopoulos A, Shimbara A, De Nadai P, Aldewachi O, Lamblin C, Lassalle P et al. Involvement of IL-9 in the bronchial phenotype of patients with nasal polyposis. Journal of Allergy and Clinical Immunology. 2004 Mar 1;113(3):462-469. https://doi.org/10.1016/j.jaci.2003.12.009
Tsicopoulos, Anne ; Shimbara, Ayako ; De Nadai, Patricia ; Aldewachi, Oday ; Lamblin, Catherine ; Lassalle, Philippe ; Walls, Andrew F. ; Sénéchal, Stéphanie ; Levitt, Roy C ; Darras, Jean ; Hamid, Qutayba ; Wallaert, Benoît. / Involvement of IL-9 in the bronchial phenotype of patients with nasal polyposis. In: Journal of Allergy and Clinical Immunology. 2004 ; Vol. 113, No. 3. pp. 462-469.
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abstract = "Background: Nasal polyposis (NP) is frequently associated with asthma. In this disease, asymptomatic bronchial hyperresponsiveness (BHR) is thought to precede the development of asthma. IL-9 and its receptor have been reported as candidate genes for asthma and to be associated with BHR. Objective: The objective of this study was to assess the contribution of IL-9 to the pathogenesis of BHR in NP by comparing the expression of IL-9 and its receptor in bronchial biopsy specimens from three groups of patients with NP:NP without BHR, NP with asymptomatic BHR, and NP with BHR and asthma. Methods: Bronchial biopsy specimens were examined in terms of cellular infiltration and in terms of expression of IL-9 protein and mRNA as well as of its receptor by using immunohistochemistry and in situ hybridization. Results: Patients with NP with asthma as compared with the two other groups exhibited an increased bronchial infiltration of basophils, eosinophils, and T cells that correlated with the asthma score. The two groups of patients with NP with BHR showed an increased expression in IL-9 protein and mRNA as well as an increase in the expression of IL-9R mRNA at the epithelial level. These modifications were inversely correlated with the airway responsiveness to methacholine, producing a 20{\%} fall in FEV1. There was a close association between IL-9+ cells, IL-5 mRNA expression, and eosinophil infiltration that correlated with each other. Conclusions: These results suggest an important role for IL-9 in the pathogenesis of BHR and a causal relation between IL-9 and the development of bronchial eosinophilia in asthma.",
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AU - Shimbara, Ayako

AU - De Nadai, Patricia

AU - Aldewachi, Oday

AU - Lamblin, Catherine

AU - Lassalle, Philippe

AU - Walls, Andrew F.

AU - Sénéchal, Stéphanie

AU - Levitt, Roy C

AU - Darras, Jean

AU - Hamid, Qutayba

AU - Wallaert, Benoît

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N2 - Background: Nasal polyposis (NP) is frequently associated with asthma. In this disease, asymptomatic bronchial hyperresponsiveness (BHR) is thought to precede the development of asthma. IL-9 and its receptor have been reported as candidate genes for asthma and to be associated with BHR. Objective: The objective of this study was to assess the contribution of IL-9 to the pathogenesis of BHR in NP by comparing the expression of IL-9 and its receptor in bronchial biopsy specimens from three groups of patients with NP:NP without BHR, NP with asymptomatic BHR, and NP with BHR and asthma. Methods: Bronchial biopsy specimens were examined in terms of cellular infiltration and in terms of expression of IL-9 protein and mRNA as well as of its receptor by using immunohistochemistry and in situ hybridization. Results: Patients with NP with asthma as compared with the two other groups exhibited an increased bronchial infiltration of basophils, eosinophils, and T cells that correlated with the asthma score. The two groups of patients with NP with BHR showed an increased expression in IL-9 protein and mRNA as well as an increase in the expression of IL-9R mRNA at the epithelial level. These modifications were inversely correlated with the airway responsiveness to methacholine, producing a 20% fall in FEV1. There was a close association between IL-9+ cells, IL-5 mRNA expression, and eosinophil infiltration that correlated with each other. Conclusions: These results suggest an important role for IL-9 in the pathogenesis of BHR and a causal relation between IL-9 and the development of bronchial eosinophilia in asthma.

AB - Background: Nasal polyposis (NP) is frequently associated with asthma. In this disease, asymptomatic bronchial hyperresponsiveness (BHR) is thought to precede the development of asthma. IL-9 and its receptor have been reported as candidate genes for asthma and to be associated with BHR. Objective: The objective of this study was to assess the contribution of IL-9 to the pathogenesis of BHR in NP by comparing the expression of IL-9 and its receptor in bronchial biopsy specimens from three groups of patients with NP:NP without BHR, NP with asymptomatic BHR, and NP with BHR and asthma. Methods: Bronchial biopsy specimens were examined in terms of cellular infiltration and in terms of expression of IL-9 protein and mRNA as well as of its receptor by using immunohistochemistry and in situ hybridization. Results: Patients with NP with asthma as compared with the two other groups exhibited an increased bronchial infiltration of basophils, eosinophils, and T cells that correlated with the asthma score. The two groups of patients with NP with BHR showed an increased expression in IL-9 protein and mRNA as well as an increase in the expression of IL-9R mRNA at the epithelial level. These modifications were inversely correlated with the airway responsiveness to methacholine, producing a 20% fall in FEV1. There was a close association between IL-9+ cells, IL-5 mRNA expression, and eosinophil infiltration that correlated with each other. Conclusions: These results suggest an important role for IL-9 in the pathogenesis of BHR and a causal relation between IL-9 and the development of bronchial eosinophilia in asthma.

KW - Asthma

KW - Bronchial biopsy

KW - Bronchial hyperresponsiveness

KW - Interleukin-9

KW - Nasal polyposis

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