Involvement of CCR-2 chemokine receptor activation in ischemic preconditioning and postconditioning of brain in mice

Ashish K. Rehni, Thakur Gurjeet Singh

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

The present study has been designed to investigate the potential role of CCR-2 chemokine receptor in ischemic preconditioning as well as postconditioning induced reversal of ischemia-reperfusion injury in mouse brain. Bilateral carotid artery occlusion of 17. min followed by reperfusion for 24. h was employed in present study to produce ischemia and reperfusion induced cerebral injury in mice. Cerebral infarct size was measured using triphenyltetrazolium chloride staining. Memory was evaluated using elevated plus-maze test and Morris water maze test. Rota rod test was employed to assess motor incoordination. Bilateral carotid artery occlusion followed by reperfusion produced cerebral infarction and impaired memory and motor co-ordination. Three preceding episodes of bilateral carotid artery occlusion for 1. min and reperfusion of 1. min were employed to elicit ischemic preconditioning of brain, while three episodes of bilateral carotid artery occlusion for 10. s and reperfusion of 10. s immediately after the completion of were employed to elicit ischemic postconditioning of brain. Both prior ischemic preconditioning as well as ischemic postconditioning immediately after global cerebral ischemia prevented markedly ischemia-reperfusion-induced cerebral injury as measured in terms of infarct size, loss of memory and motor coordination. RS 102895, a selective CCR-2 chemokine receptor antagonist, attenuated the neuroprotective effect of both the ischemic preconditioning as well as postconditioning. It is concluded that the neuroprotective effect of both ischemic preconditioning as well as ischemic postconditioning may involve the activation of CCR-2 chemokine receptors.

Original languageEnglish (US)
Pages (from-to)83-89
Number of pages7
JournalCytokine
Volume60
Issue number1
DOIs
StatePublished - Oct 2012
Externally publishedYes

Keywords

  • CCR-2 chemokine receptors
  • Cerebral ischemia
  • Ischemic postconditioning
  • Ischemic preconditioning
  • RS 102895

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

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