In Vivo Characteristics of Corneal Endothelium/Descemet Membrane Complex for the Diagnosis of Corneal Graft Rejection

Mohamed Abou Shousha, Sonia H Yoo, Mohamed S. Sayed, Sean Edelstein, Matthew Council, Ravi S. Shah, Joshua Abernathy, Zachary Schmitz, Patrick Stuart, Rocio Bentivegna, Maria P. Fernandez, Christopher Smith, Xiaotang Yin, George J. Harocopos, Sander Dubovy, William J Feuer, Jianhua Wang, Victor L Perez Quinones

Research output: Contribution to journalArticle

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Abstract

Purpose To evaluate the utility of endothelial/Descemet membrane complex (En/DM) characteristics in diagnosing corneal graft rejection. Design Diagnostic reliability study. Methods One hundred thirty-nine eyes (96 corneal grafts post penetrating keratoplasty or Descemet stripping automated endothelial keratoplasty: 40 clear, 23 actively rejecting, 24 rejected, and 9 nonimmunologic failed grafts; along with 43 age-matched control eyes) were imaged using high-definition optical coherence tomography. Images were used to describe En/DM and measure central corneal thickness (CCT) and central En/DM thickness (DMT). En/DM rejection index (DRI) was computed to detect the relative En/DM thickening to the entire cornea. Results In actively rejecting grafts, DMT and DRI were significantly greater than controls and clear grafts (28, 17, and 17 μm and 1.5, 1 and 1, respectively; P < .001). Rejected grafts had the highest DMT and DRI compared to all groups (59 μm and 2.1; P < .001). DMT and DRI showed excellent accuracy, significantly better than that of CCT, in differentiating actively rejecting from clear grafts (100% and 96% sensitivity; 92.5% and 92.5% specificity), actively rejecting from rejected grafts (88% and 83% sensitivity; 91% and 83% specificity), and nonimmunologic failed from rejected grafts (100% and 100% sensitivity; 88% and 100% specificity). DMT correlated significantly with rejection severity (P < .001). Conclusions In corneal grafts, in vivo relative thickening of the En/DM is diagnostic of graft rejection as measured by DMT and DRI. These indices have excellent accuracy, sensitivity, and specificity in detecting graft immunologic status, superior to CCT. DMT is a quantitative index that correlates accurately with the severity of rejection.

Original languageEnglish (US)
Pages (from-to)27-37
Number of pages11
JournalAmerican Journal of Ophthalmology
Volume178
DOIs
StatePublished - Jun 1 2017

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Descemet Membrane
Corneal Endothelium
Graft Rejection
Transplants
Descemet Stripping Endothelial Keratoplasty
Penetrating Keratoplasty
Optical Coherence Tomography
Cornea

ASJC Scopus subject areas

  • Ophthalmology

Cite this

In Vivo Characteristics of Corneal Endothelium/Descemet Membrane Complex for the Diagnosis of Corneal Graft Rejection. / Abou Shousha, Mohamed; Yoo, Sonia H; Sayed, Mohamed S.; Edelstein, Sean; Council, Matthew; Shah, Ravi S.; Abernathy, Joshua; Schmitz, Zachary; Stuart, Patrick; Bentivegna, Rocio; Fernandez, Maria P.; Smith, Christopher; Yin, Xiaotang; Harocopos, George J.; Dubovy, Sander; Feuer, William J; Wang, Jianhua; Perez Quinones, Victor L.

In: American Journal of Ophthalmology, Vol. 178, 01.06.2017, p. 27-37.

Research output: Contribution to journalArticle

Abou Shousha, M, Yoo, SH, Sayed, MS, Edelstein, S, Council, M, Shah, RS, Abernathy, J, Schmitz, Z, Stuart, P, Bentivegna, R, Fernandez, MP, Smith, C, Yin, X, Harocopos, GJ, Dubovy, S, Feuer, WJ, Wang, J & Perez Quinones, VL 2017, 'In Vivo Characteristics of Corneal Endothelium/Descemet Membrane Complex for the Diagnosis of Corneal Graft Rejection', American Journal of Ophthalmology, vol. 178, pp. 27-37. https://doi.org/10.1016/j.ajo.2017.02.026
Abou Shousha, Mohamed ; Yoo, Sonia H ; Sayed, Mohamed S. ; Edelstein, Sean ; Council, Matthew ; Shah, Ravi S. ; Abernathy, Joshua ; Schmitz, Zachary ; Stuart, Patrick ; Bentivegna, Rocio ; Fernandez, Maria P. ; Smith, Christopher ; Yin, Xiaotang ; Harocopos, George J. ; Dubovy, Sander ; Feuer, William J ; Wang, Jianhua ; Perez Quinones, Victor L. / In Vivo Characteristics of Corneal Endothelium/Descemet Membrane Complex for the Diagnosis of Corneal Graft Rejection. In: American Journal of Ophthalmology. 2017 ; Vol. 178. pp. 27-37.
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abstract = "Purpose To evaluate the utility of endothelial/Descemet membrane complex (En/DM) characteristics in diagnosing corneal graft rejection. Design Diagnostic reliability study. Methods One hundred thirty-nine eyes (96 corneal grafts post penetrating keratoplasty or Descemet stripping automated endothelial keratoplasty: 40 clear, 23 actively rejecting, 24 rejected, and 9 nonimmunologic failed grafts; along with 43 age-matched control eyes) were imaged using high-definition optical coherence tomography. Images were used to describe En/DM and measure central corneal thickness (CCT) and central En/DM thickness (DMT). En/DM rejection index (DRI) was computed to detect the relative En/DM thickening to the entire cornea. Results In actively rejecting grafts, DMT and DRI were significantly greater than controls and clear grafts (28, 17, and 17 μm and 1.5, 1 and 1, respectively; P < .001). Rejected grafts had the highest DMT and DRI compared to all groups (59 μm and 2.1; P < .001). DMT and DRI showed excellent accuracy, significantly better than that of CCT, in differentiating actively rejecting from clear grafts (100{\%} and 96{\%} sensitivity; 92.5{\%} and 92.5{\%} specificity), actively rejecting from rejected grafts (88{\%} and 83{\%} sensitivity; 91{\%} and 83{\%} specificity), and nonimmunologic failed from rejected grafts (100{\%} and 100{\%} sensitivity; 88{\%} and 100{\%} specificity). DMT correlated significantly with rejection severity (P < .001). Conclusions In corneal grafts, in vivo relative thickening of the En/DM is diagnostic of graft rejection as measured by DMT and DRI. These indices have excellent accuracy, sensitivity, and specificity in detecting graft immunologic status, superior to CCT. DMT is a quantitative index that correlates accurately with the severity of rejection.",
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T1 - In Vivo Characteristics of Corneal Endothelium/Descemet Membrane Complex for the Diagnosis of Corneal Graft Rejection

AU - Abou Shousha, Mohamed

AU - Yoo, Sonia H

AU - Sayed, Mohamed S.

AU - Edelstein, Sean

AU - Council, Matthew

AU - Shah, Ravi S.

AU - Abernathy, Joshua

AU - Schmitz, Zachary

AU - Stuart, Patrick

AU - Bentivegna, Rocio

AU - Fernandez, Maria P.

AU - Smith, Christopher

AU - Yin, Xiaotang

AU - Harocopos, George J.

AU - Dubovy, Sander

AU - Feuer, William J

AU - Wang, Jianhua

AU - Perez Quinones, Victor L

PY - 2017/6/1

Y1 - 2017/6/1

N2 - Purpose To evaluate the utility of endothelial/Descemet membrane complex (En/DM) characteristics in diagnosing corneal graft rejection. Design Diagnostic reliability study. Methods One hundred thirty-nine eyes (96 corneal grafts post penetrating keratoplasty or Descemet stripping automated endothelial keratoplasty: 40 clear, 23 actively rejecting, 24 rejected, and 9 nonimmunologic failed grafts; along with 43 age-matched control eyes) were imaged using high-definition optical coherence tomography. Images were used to describe En/DM and measure central corneal thickness (CCT) and central En/DM thickness (DMT). En/DM rejection index (DRI) was computed to detect the relative En/DM thickening to the entire cornea. Results In actively rejecting grafts, DMT and DRI were significantly greater than controls and clear grafts (28, 17, and 17 μm and 1.5, 1 and 1, respectively; P < .001). Rejected grafts had the highest DMT and DRI compared to all groups (59 μm and 2.1; P < .001). DMT and DRI showed excellent accuracy, significantly better than that of CCT, in differentiating actively rejecting from clear grafts (100% and 96% sensitivity; 92.5% and 92.5% specificity), actively rejecting from rejected grafts (88% and 83% sensitivity; 91% and 83% specificity), and nonimmunologic failed from rejected grafts (100% and 100% sensitivity; 88% and 100% specificity). DMT correlated significantly with rejection severity (P < .001). Conclusions In corneal grafts, in vivo relative thickening of the En/DM is diagnostic of graft rejection as measured by DMT and DRI. These indices have excellent accuracy, sensitivity, and specificity in detecting graft immunologic status, superior to CCT. DMT is a quantitative index that correlates accurately with the severity of rejection.

AB - Purpose To evaluate the utility of endothelial/Descemet membrane complex (En/DM) characteristics in diagnosing corneal graft rejection. Design Diagnostic reliability study. Methods One hundred thirty-nine eyes (96 corneal grafts post penetrating keratoplasty or Descemet stripping automated endothelial keratoplasty: 40 clear, 23 actively rejecting, 24 rejected, and 9 nonimmunologic failed grafts; along with 43 age-matched control eyes) were imaged using high-definition optical coherence tomography. Images were used to describe En/DM and measure central corneal thickness (CCT) and central En/DM thickness (DMT). En/DM rejection index (DRI) was computed to detect the relative En/DM thickening to the entire cornea. Results In actively rejecting grafts, DMT and DRI were significantly greater than controls and clear grafts (28, 17, and 17 μm and 1.5, 1 and 1, respectively; P < .001). Rejected grafts had the highest DMT and DRI compared to all groups (59 μm and 2.1; P < .001). DMT and DRI showed excellent accuracy, significantly better than that of CCT, in differentiating actively rejecting from clear grafts (100% and 96% sensitivity; 92.5% and 92.5% specificity), actively rejecting from rejected grafts (88% and 83% sensitivity; 91% and 83% specificity), and nonimmunologic failed from rejected grafts (100% and 100% sensitivity; 88% and 100% specificity). DMT correlated significantly with rejection severity (P < .001). Conclusions In corneal grafts, in vivo relative thickening of the En/DM is diagnostic of graft rejection as measured by DMT and DRI. These indices have excellent accuracy, sensitivity, and specificity in detecting graft immunologic status, superior to CCT. DMT is a quantitative index that correlates accurately with the severity of rejection.

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