Investigation of the electroosmotic flow effect on the efficiency of capillary electrophoresis

Victor P. Andreev, E. E. Lisin

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

The influence of the electroosmotic flow profile on the efficiency and resolution of capillary electrophoresis is studied. The mathematical model is formulated and the set of equations is solved numerically. The results of the analysis are applicable to a wide range of buffer concentrations and capillary diameters. The temperature dependence of electrophoretic mobility, viscosity and thermal conductivity and the dependence of electrical conductivity on temperature and ion concentration in the double layer are taken into account. It is shown that there exists a region of buffer concentrations and capillary diameters where the influence of the electroosmotic flow profile on the efficiency and resolution is much greater than that of the temperature dependence of the electrophoretic mobility. The results are especially essential for small buffer concentrations or capillary diameters comparable with the double electrical layer thickness.

Original languageEnglish (US)
Pages (from-to)832-837
Number of pages6
JournalELECTROPHORESIS
Volume13
Issue number1
DOIs
StatePublished - 1992

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

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