Investigation of the electroosmotic flow effect on the efficiency of capillary electrophoresis

Victor P. Andreev; E. E. Lisin

doi:10.1002/elps.11501301183

Investigation of the electroosmotic flow effect on the efficiency of capillary electrophoresis

Victor P. Andreev, E. E. Lisin

Psychiatry & Behavioral Sciences

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

The influence of the electroosmotic flow profile on the efficiency and resolution of capillary electrophoresis is studied. The mathematical model is formulated and the set of equations is solved numerically. The results of the analysis are applicable to a wide range of buffer concentrations and capillary diameters. The temperature dependence of electrophoretic mobility, viscosity and thermal conductivity and the dependence of electrical conductivity on temperature and ion concentration in the double layer are taken into account. It is shown that there exists a region of buffer concentrations and capillary diameters where the influence of the electroosmotic flow profile on the efficiency and resolution is much greater than that of the temperature dependence of the electrophoretic mobility. The results are especially essential for small buffer concentrations or capillary diameters comparable with the double electrical layer thickness.

Original language	English (US)
Pages (from-to)	832-837
Number of pages	6
Journal	ELECTROPHORESIS
Volume	13
Issue number	1
DOIs	https://doi.org/10.1002/elps.11501301183
State	Published - 1992

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry

Access to Document

10.1002/elps.11501301183

Fingerprint Dive into the research topics of 'Investigation of the electroosmotic flow effect on the efficiency of capillary electrophoresis'. Together they form a unique fingerprint.

Cite this

@article{50026d4331834964a5cac2fc7520a73f,

title = "Investigation of the electroosmotic flow effect on the efficiency of capillary electrophoresis",

abstract = "The influence of the electroosmotic flow profile on the efficiency and resolution of capillary electrophoresis is studied. The mathematical model is formulated and the set of equations is solved numerically. The results of the analysis are applicable to a wide range of buffer concentrations and capillary diameters. The temperature dependence of electrophoretic mobility, viscosity and thermal conductivity and the dependence of electrical conductivity on temperature and ion concentration in the double layer are taken into account. It is shown that there exists a region of buffer concentrations and capillary diameters where the influence of the electroosmotic flow profile on the efficiency and resolution is much greater than that of the temperature dependence of the electrophoretic mobility. The results are especially essential for small buffer concentrations or capillary diameters comparable with the double electrical layer thickness.",

author = "Andreev, {Victor P.} and Lisin, {E. E.}",

year = "1992",

doi = "10.1002/elps.11501301183",

language = "English (US)",

volume = "13",

pages = "832--837",

journal = "Electrophoresis",

issn = "0173-0835",

publisher = "Wiley-VCH Verlag",

number = "1",

}

TY - JOUR

T1 - Investigation of the electroosmotic flow effect on the efficiency of capillary electrophoresis

AU - Andreev, Victor P.

AU - Lisin, E. E.

PY - 1992

Y1 - 1992

N2 - The influence of the electroosmotic flow profile on the efficiency and resolution of capillary electrophoresis is studied. The mathematical model is formulated and the set of equations is solved numerically. The results of the analysis are applicable to a wide range of buffer concentrations and capillary diameters. The temperature dependence of electrophoretic mobility, viscosity and thermal conductivity and the dependence of electrical conductivity on temperature and ion concentration in the double layer are taken into account. It is shown that there exists a region of buffer concentrations and capillary diameters where the influence of the electroosmotic flow profile on the efficiency and resolution is much greater than that of the temperature dependence of the electrophoretic mobility. The results are especially essential for small buffer concentrations or capillary diameters comparable with the double electrical layer thickness.

AB - The influence of the electroosmotic flow profile on the efficiency and resolution of capillary electrophoresis is studied. The mathematical model is formulated and the set of equations is solved numerically. The results of the analysis are applicable to a wide range of buffer concentrations and capillary diameters. The temperature dependence of electrophoretic mobility, viscosity and thermal conductivity and the dependence of electrical conductivity on temperature and ion concentration in the double layer are taken into account. It is shown that there exists a region of buffer concentrations and capillary diameters where the influence of the electroosmotic flow profile on the efficiency and resolution is much greater than that of the temperature dependence of the electrophoretic mobility. The results are especially essential for small buffer concentrations or capillary diameters comparable with the double electrical layer thickness.

UR - http://www.scopus.com/inward/record.url?scp=0026747251&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026747251&partnerID=8YFLogxK

U2 - 10.1002/elps.11501301183

DO - 10.1002/elps.11501301183

M3 - Article

C2 - 1483424

AN - SCOPUS:0026747251

VL - 13

SP - 832

EP - 837

JO - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

IS - 1

ER -