Investigation of the electroosmotic flow effect on the efficiency of capillary electrophoresis

Victor P. Andreev; E. E. Lisin

doi:10.1002/elps.11501301183

Investigation of the electroosmotic flow effect on the efficiency of capillary electrophoresis

Victor P. Andreev, E. E. Lisin

Psychiatry & Behavioral Sciences

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

The influence of the electroosmotic flow profile on the efficiency and resolution of capillary electrophoresis is studied. The mathematical model is formulated and the set of equations is solved numerically. The results of the analysis are applicable to a wide range of buffer concentrations and capillary diameters. The temperature dependence of electrophoretic mobility, viscosity and thermal conductivity and the dependence of electrical conductivity on temperature and ion concentration in the double layer are taken into account. It is shown that there exists a region of buffer concentrations and capillary diameters where the influence of the electroosmotic flow profile on the efficiency and resolution is much greater than that of the temperature dependence of the electrophoretic mobility. The results are especially essential for small buffer concentrations or capillary diameters comparable with the double electrical layer thickness.

Original language	English (US)
Pages (from-to)	832-837
Number of pages	6
Journal	ELECTROPHORESIS
Volume	13
Issue number	1
DOIs	https://doi.org/10.1002/elps.11501301183
State	Published - 1992

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry

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AB - The influence of the electroosmotic flow profile on the efficiency and resolution of capillary electrophoresis is studied. The mathematical model is formulated and the set of equations is solved numerically. The results of the analysis are applicable to a wide range of buffer concentrations and capillary diameters. The temperature dependence of electrophoretic mobility, viscosity and thermal conductivity and the dependence of electrical conductivity on temperature and ion concentration in the double layer are taken into account. It is shown that there exists a region of buffer concentrations and capillary diameters where the influence of the electroosmotic flow profile on the efficiency and resolution is much greater than that of the temperature dependence of the electrophoretic mobility. The results are especially essential for small buffer concentrations or capillary diameters comparable with the double electrical layer thickness.

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Investigation of the electroosmotic flow effect on the efficiency of capillary electrophoresis

Abstract

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